Mouse Intelectin-2 Antibody

Catalog #: MAB5358 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5358
MAB5358-SP

Detection of Mouse and Rat Intelectin‑2 by Western Blot.

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All Intelectin-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Intelectin-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) Intelectin-1 is observed. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) Intelectin-2 is observed, and approximately 15% cross-reactivity with rhIntelectin-1 is observed.

Source

Monoclonal Rat IgG₁ Clone # 747421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Intelectin‑2 in Mouse Intestine. Intelectin-2 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-2 Monoclonal Antibody (Catalog # MAB5358) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (endothelial lectin 2; also ITLN-2 and HL-2) is a 40 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells and appears to participate in fungal microbe recognition. The Intelectin-2 precursor is 325 amino acids (aa) in length. It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse Intelectin-2 is reportedly GPI-linked and mouse and human Intelectin-2 show considerable C‑terminal aa identity, it is not known if human Intelectin-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 45‑210). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 20‑298, mouse Intelectin-2 shows 78% aa identity to human Intelectin-2.

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Alternate Names

HL-2; intelectin 2; Intelectin2; Intelectin-2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179