Mouse Intelectin-2 Antibody

Catalog #: AF5358 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5358
AF5358-SP

Detection of Mouse Intelectin‑2 by Western Blot.

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All Intelectin-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Intelectin-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Intelectin-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Intelectin-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Intelectin-2
Ala20-Ser298
Accession # Q80ZA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Intelectin-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-2

Intelectin-2 (ITLN-2, ITLN-1b and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by airway mucous cells plus intestinal Paneth and goblet cells, and appears to participate in fungal microbe recognition. The mouse ITLN-2 precursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature region (aa 20-298), and a GPI-linked prodomain (aa 299-313). The mature region contains one C-terminal type fibrinogen domain (aa 32-251). Over aa 20-298, mouse ITLN-2 shares 79% aa sequence identity with human ITLN-2, and 92% aa sequence identity with mouse ITLN-1.

Entrez Gene IDs

142683 (Human); 493583 (Mouse)

Alternate Names

HL-2; intelectin 2; Intelectin2; Intelectin-2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179