Mouse IRF5 PE-conjugated Antibody Summary
Ala208-Phe288
Accession # P56477
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IRF5 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse IRF5 PE-conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Detection of IRF5 in A20 cells by Flow Cytometry A20 cells were stained with Rat Anti-Mouse IRF5 PE‑conjugated Monoclonal Antibody (Catalog # IC8447P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IRF5
IRF5 is a member of the IRF family of transcription factors, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF5, along with IRF7, are the key mediators of TLR signaling. IRF5 forms heterodimers with IRF3 both of which are necessary for interferon gene transcription. IRF5 knock out mice indicate that IRF5 is critical for induction of apoptosis.
Product Datasheets
Citations for Mouse IRF5 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Investigating Chemokine-Matrix Networks in Breast Cancer: Tenascin-C Sets the Tone for CCL2
Authors: Gschwandtner, M;Gammage, AN;Deligne, C;Mies, LFM;Domaingo, A;Murdamoothoo, D;Loustau, T;Schwenzer, A;Derler, R;Carapito, R;Koch, M;M�rgelin, M;Orend, G;Kungl, AJ;Midwood, KS;
International journal of molecular sciences
Species: Mouse
Sample Types: Whole Cells
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Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality
Sci Rep, 2016-08-02;6(0):31002.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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