Mouse ISLR-2 Antibody Summary
Cys20-Leu590
Accession # Q5RKR3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse ISLR‑2 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ISLR-2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
View Larger
ISLR‑2 in Embryonic Mouse Brainstem. ISLR-2 was detected in immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.) using Sheep Anti-Mouse ISLR-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ISLR-2
ISLR2 (Ig‑superfamily containing LRR protein 2; also SST273) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mouse ISLR2 is 745 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 132 aa cytoplasmic region. The ECD shows multiple LRRs (aa 21‑231) and one Ig‑like domain (aa 251‑374). Mouse ISLR2 apparently has one alternate start site at Met130. Over aa 20‑590, mouse ISLR2 is 89% and 98% aa identical to human and rat ISLR2, respectively.
Product Datasheets
Citations for Mouse ISLR-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
Nat Commun, 2020-10-14;11(1):5171.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure
Authors: S Abudureyim, N Asai, A Enomoto, L Weng, H Kobayashi, X Wang, C Chen, S Mii, M Takahashi
Sci Rep, 2018-05-08;8(1):7292.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse ISLR-2 Antibody
There are currently no reviews for this product. Be the first to review Mouse ISLR-2 Antibody and earn rewards!
Have you used Mouse ISLR-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
