Home / JAM-B/VE-JAM / Mouse JAM-B/VE-JAM Antibody

Mouse JAM-B/VE-JAM Antibody

Catalog #: MAB9881
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9881-SP
MAB9881-100
MAB9881-500
Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutral-ization by Mouse JAM‑B/ VE‑JAM Antibody.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse JAM-B/VE-JAM Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse JAM‑B/VE-JAM in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human JAM-1, -2, or -3 is observed.
Source
Monoclonal Rat IgG2A Clone # 150005
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM‑B/VE-JAM
Phe29-Asn236 (predicted)
Accession # Q9JI59
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize JAM‑B/VE‑JAM-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse JAM‑B/VE‑JAM Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutral-ization by Mouse JAM‑B/ VE‑JAM Antibody. View Larger

Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutral-ization by Mouse JAM‑B/ VE‑JAM Antibody. Recombinant Mouse JAM-B/VE-JAM Fc Chimera (Catalog # 988-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse JAM-B/VE-JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse JAM-B/ VE-JAM Monoclonal Antibody (Catalog # MAB9881). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-B/VE-JAM

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (2, 3, 5). Mouse JAM-B/VE-JAM cDNA predicts a 298 amino acid (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (2, 3). Mouse JAM-B shares approximately 79% aa sequence homology with its human homologue. It also shares approximately 35% aa sequence homology with mouse JAM-A or JAM‑C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM‑C, but not JAM-A (4, 5, 7). It is also a ligand for the Integrin alpha4beta1. However, the JAM-B/alpha4beta1 interaction is facilitated only after prior adhesion of JAM-B to JAM‑C (6). Through its heterotypic interactions with JAM‑C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. 
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
  4. Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
  5. Liang, T. et al. (2002) J. Immunol. 168:1618.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.
Long Name
Junctional Adhesion Molecule B
Entrez Gene IDs
58494 (Human); 67374 (Mouse)
Alternate Names
C21orf43; CD322 antigen; CD322; JAM2; JAMB; JAM-B; JAM-BJAM-IT/VE-JAM; junctional adhesion molecule 2JAM-2; junctional adhesion molecule B; PRO245; Vascular endothelial junction-associated molecule; VE-JAM; VE-JAMVEJAMCD322

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse JAM-B/VE-JAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination
    Neuron, 2016-08-04;91(4):824-836.
  2. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin.
    Authors: Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
    Eur J Immunol, 2015-10-06;45(12):3302-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009-10-01;128(2):196-205.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse JAM-B/VE-JAM Antibody

There are currently no reviews for this product. Be the first to review Mouse JAM-B/VE-JAM Antibody and earn rewards!

Have you used Mouse JAM-B/VE-JAM Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review