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Mouse JAM-C Antibody

Catalog #: AF1213
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1213
AF1213-SP
JAM-C in Mouse Embryo.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse JAM-C Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM‑C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse JAM-C
Val32-Asn241
Accession # Q9D8B7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse JAM-C Fc Chimera (Catalog # 1213-J3)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-15 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Mouse VEJAM to immobilized Recombinant Mouse JAM-C (Catalog # 1213-J3) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry JAM-C antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

JAM-C in Mouse Embryo. JAM-C was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry JAM-C antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

JAM‑C in Mouse Splenocytes. JAM-C was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1213) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-C

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.
  3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.
  4. Johnson-Leger, C. et al. (2002) Blood 100:25793.
Long Name
Junctional Adhesion Molecule C
Entrez Gene IDs
83700 (Human); 83964 (Mouse)
Alternate Names
CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C

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Citations for Mouse JAM-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development
    Authors: Sun, Q;Li, J;Ma, J;Zheng, Y;Ju, R;Li, X;Ren, X;Huang, L;Chen, R;Tan, X;Luo, L;
    Investigative ophthalmology & visual science
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Macrophage fusion event as one prerequisite for inorganic nanoparticle-induced antitumor response
    Authors: Chen S, Xing Z, Geng M et al.
    Science advances
  3. ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling
    Authors: N McMillan, GW Kirschen, S Desai, E Xia, SE Tsirka, A Aguirre
    Neural regeneration research, 2022-11-01;17(11):2472-2483.
    Species: Mouse
    Sample Types: Tissue Lysates, Whole Cells
    Applications: IHC, Immunoprecipitation, Neutralization
  4. Deficiency of Jamc Leads to Congenital Nuclear Cataract and Activates the Unfolded Protein Response in Mouse Lenses
    Authors: J Li, X Tan, Q Sun, X Li, R Chen, L Luo
    Investigative Ophthalmology & Visual Science, 2022-09-01;63(10):1.
    Species: Mouse
    Sample Types: Tissue Lysate
  5. Junctional Adhesion Molecule 3 Expression in the Mouse Airway Epithelium Is Linked to Multiciliated Cells
    Authors: Clara Maria Mateos-Quiros, Sergio Garrido-Jimenez, Guadalupe Álvarez-Hernán, Selene Diaz-Chamorro, Juan Francisco Barrera-Lopez, Javier Francisco-Morcillo et al.
    Frontiers in Cell and Developmental Biology
  6. Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells
    Authors: Morio Yamazaki, Kotaro Sugimoto, Yo Mabuchi, Rina Yamashita, Naoki Ichikawa-Tomikawa, Tetsuharu Kaneko et al.
    Biomedicines
  7. Neutrophil‐endothelial interactions of murine cells is not a good predictor of their interactions in human cells
    Authors: Fariborz Soroush, Yuan Tang, Omar Mustafa, Shuang Sun, Qingliang Yang, Laurie E. Kilpatrick et al.
    The FASEB Journal
  8. Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis
    Authors: Amandine Cartier-Michaud, Anne-Laure Bailly, Stéphane Betzi, Xiaoli Shi, Jean-Claude Lissitzky, Ana Zarubica et al.
    PLOS Genetics
  9. Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
    Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
    Cell Adh Migr, 2016-04-25;10(4):419-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Junctional adhesion molecule (JAM)-C deficient C57BL/6 mice develop a severe hydrocephalus.
    Authors: Wyss L, Schafer J, Liebner S, Mittelbronn M, Deutsch U, Enzmann G, Adams R, Aurrand-Lions M, Plate K, Imhof B, Engelhardt B
    PLoS ONE, 2012-09-18;7(9):e45619.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
    Authors: Economopoulou M, Hammer J, Wang F, Fariss R, Maminishkis A, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2008-12-05;50(3):1454-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
    Authors: Fuse C, Ishida Y, Hikita T, Asai T, Oku N
    J. Biol. Chem., 2007-01-16;282(11):8276-83.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
    Authors: Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M
    J. Invest. Dermatol., 2005-11-01;125(5):969-76.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization

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