Mouse JMJD6/PSR Antibody

Catalog #: AF1994 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1994
AF1994-SP

All JMJD6/PSR Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse JMJD6/PSR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse JMJD6/PSR in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JMJD6/PSR
Ala289-Arg403
Accession # Q9ERI5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse JMJD6/PSR

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JMJD6/PSR

JMJD6 (Jumonji [Japanese for "cruciform/cross"] domain-containing protein 6; also PTDSR/PSR and Lysyl-hydroxylase JMJD6) is a 47-55 kDa member of the JMJD6 family, cupin superfamily of proteins. It is induced by hypoxia, and expressed in skeletal muscle, macrophages and possibly endothelial cells. JMJD6 is reported to demethylate histones (which it does not), serve as a phosphatidyl serine receptor (which it might), and act as a lysyl hydroxylase (which it does). In the nucleus, it appears to regulate pre-RNA splicing by hydroxylating various splicing factors such as U2AF65. Mouse JMJD6 is 403 amino acids (aa) in length. It contains five NLS's plus a JmjC domain (aa 141-305) and three iron-based catalytic sites. There at least three potential isoform variants. One contains a 29 aa substitution for aa 270‑403, a second possesses a 30 aa substitution for aa 302-314, and a third shows a 21 aa substitution for aa 315-403. JMJD6 is known to form high MW homo‑trimers, -tetramers and -oligomers. Full-length mouse JMJD6 shares 98% and 99% aa sequence identity with human and rat JMJD6, respectively.

Long Name

Jumonji Domain Containing 6/Phosphatidylserine Receptor

Entrez Gene IDs

23210 (Human); 107817 (Mouse); 360665 (Rat)

Alternate Names

bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; EC 1.14.11; EC 1.14.11.-; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; JMJD6; jumonji domain containing 6; Jumonji domain-containing protein 6; KIAA0585PSR; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptorPTDSR; Protein PTDSR; PSR; PTDSR; PTDSR1