Home / Ki67/MKI67 / Mouse Ki67/MKI67 Antibody

Mouse Ki67/MKI67 Antibody

Catalog #: AF7649
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7649
AF7649-SP
Ki67/MKI67 in NIH‑3T3 Mouse Cell Line.
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Citations (28)
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Mouse Ki67/MKI67 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ki67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki67/MKI67 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Ki67/MKI67
Asn3053-Ser3177
Accession # NP_001074586
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Ki67/MKI67 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Ki67/MKI67 in NIH‑3T3 Mouse Cell Line. Ki67/MKI67 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ki67/MKI67

MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.

Long Name
Antigen Identified by Monoclonal Antibody Ki67
Entrez Gene IDs
4288 (Human); 17345 (Mouse); 291234 (Rat)
Alternate Names
antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126

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Citations for Mouse Ki67/MKI67 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial Cells
    Authors: Yung-Yu Yang, Chao-Ju Lin, Cheng-Chin Wang, Chieh-Min Chen, Wen-Jen Kao, Yi-Hui Chen
    Frontiers in Cell and Developmental Biology
  2. Reactive morphology of dividing microglia following kainic acid administration
    Authors: Tabitha R. F. Green, Sean M. Murphy, Maria P. Moreno-Montano, Etienne Audinat, Rachel K. Rowe
    Frontiers in Neuroscience
  3. Intranasal Delivery of lincRNA-Cox2 siRNA Loaded Extracellular Vesicles Decreases Lipopolysaccharide-Induced Microglial Proliferation in Mice
    Authors: Ke Liao, Fang Niu, Raghubendra Singh Dagur, Mengfan He, Changhai Tian, Guoku Hu
    Journal of Neuroimmune Pharmacology
  4. Lifespan of mature olfactory sensory neurons varies with location in the mouse olfactory epithelium and age of the animal
    Authors: Vera Gaun, Jeffrey R. Martens, James E. Schwob
    Journal of Comparative Neurology
  5. Mechanical Loading Promotes the Expansion of Primitive Osteoprogenitors and Organizes Matrix and Vascular Morphology in Long Bone Defects
    Authors: Chao Liu, Pamela Cabahug‐Zuckerman, Christopher Stubbs, Martin Pendola, Cinyee Cai, Kenneth A. Mann et al.
    Journal of Bone and Mineral Research
  6. Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis
    Authors: Chaube, B;Citrin, KM;Sahraei, M;Singh, AK;de Urturi, DS;Ding, W;Pierce, RW;Raaisa, R;Cardone, R;Kibbey, R;Fernández-Hernando, C;Suárez, Y;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Systemic delivery of glycosylated-PEG-masked oncolytic virus enhances targeting of antitumor immuno-virotherapy and modulates T and NK cell infiltration
    Authors: Liang, Y;Wang, B;Chen, Q;Fu, X;Jiang, C;Lin, Z;Zhuang, Q;Zeng, Y;Liu, X;Zhang, D;
    Theranostics
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  8. Itaconate-producing neutrophils regulate local and systemic inflammation following trauma
    Authors: Crossley, JL;Ostashevskaya-Gohstand, S;Comazzetto, S;Hook, JS;Guo, L;Vishlaghi, N;Juan, C;Xu, L;Horswill, AR;Hoxhaj, G;Moreland, JG;Tower, RJ;Levi, B;
    JCI insight
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Atoh1 drives the heterogeneity of the pontine nuclei neurons and promotes their differentiation
    Authors: Wu, SR;Butts, JC;Caudill, MS;Revelli, JP;Dhindsa, RS;Durham, MA;Zoghbi, HY;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Genetic recording of in vivo cell proliferation by ProTracer
    Authors: Liu, X;Weng, W;He, L;Zhou, B;
    Nature protocols
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
    Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
    bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Human Bone Marrow-Derived Mesenchymal Stromal Cells Reduce the Severity of Experimental Necrotizing Enterocolitis in a Concentration-Dependent Manner
    Authors: L Provitera, A Tomaselli, G Raffaeli, S Crippa, C Arribas, I Amodeo, S Gulden, GS Amelio, V Cortesi, F Manzoni, G Cervellini, J Cerasani, C Menis, N Pesenti, M Tripodi, L Santi, M Maggioni, C Lonati, S Oldoni, F Algieri, F Garrido, ME Bernardo, F Mosca, G Cavallaro
    Cells, 2023-02-27;12(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands
    Authors: Y Wang, ER Zoneff, JW Thomas, N Hong, LL Tan, DJ McGillivra, AW Perriman, KCL Law, LH Thompson, N Moriarty, CL Parish, RJ Williams, CJ Jackson, DR Nisbet
    Nature Communications, 2023-01-28;14(1):457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Role of ERK signaling in bladder urothelium in response to cyclophosphamide injury
    Authors: ST Narla, JL Duara, DS Bushnell, M Nouraie, J Holden, K Pfister, PC Lucas, S Sims-Lucas, CM Bates
    Physiological Reports, 2022-07-01;10(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Durability of and role of AKT in FGF7p urothelial protection against cyclophosphamide
    Authors: ST Narla, L Rice, D Ostrov, DS Bushnell, JL Duara, CM Bates
    Physiological Reports, 2022-06-01;10(12):e15358.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Hedgehog-responsive PDGFRa(+) fibroblasts maintain a unique pool of alveolar epithelial progenitor cells during alveologenesis
    Authors: F Gao, C Li, S Danopoulos, D Al Alam, N Peinado, S Webster, Z Borok, GA Kohbodi, S Bellusci, P Minoo
    Cell Reports, 2022-04-05;39(1):110608.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
    Authors: J Wang, R Hao, T Jiang, X Guo, F Zhou, L Cao, F Gao, G Wang, J Wang, K Ning, C Zhong, X Chen, Y Huang, J Xu, S Gao
    Cell & bioscience, 2022-04-04;12(1):40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide
    Authors: ST Narla, L Rice, D Ostrov, SG Swarts, DW Siemann, DS Bushnell, JG Holden, JL Duara, CM Bates
    Physiological Reports, 2022-04-01;10(7):e15241.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
    Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
    Nature Communications, 2021-12-08;12(1):7142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression
    Authors: JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold
    Science Advances, 2021-11-03;7(45):eabg9518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Reactivity and increased proliferation of NG2 cells following central nervous system infection with Theiler's murine encephalomyelitis virus
    Authors: LA Bell, GJ Wallis, KS Wilcox
    Journal of Neuroinflammation, 2020-12-03;17(1):369.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. The aging skin microenvironment dictates stem cell behavior
    Authors: Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs
    Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic ? Cell Hyperplasia in Mice
    Authors: J Kim, H Okamoto, Z Huang, G Anguiano, S Chen, Q Liu, K Cavino, Y Xin, E Na, R Hamid, J Lee, B Zambrowicz, R Unger, AJ Murphy, Y Xu, GD Yancopoulo, WH Li, J Gromada
    Cell Metab., 2017-06-06;25(6):1348-1361.e8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
    Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
    Nat Commun, 2017-03-16;8(0):14600.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis.
    Authors: Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, Thangaraju M, Prasad P, Manicassamy S, Munn D, Lee J, Offermanns S, Ganapathy V
    Immunity, 2014-01-09;40(1):128-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Efferocytosis induces macrophage proliferation to help resolve tissue injury
    Authors: Gerlach BD, Ampomah PB, Yurdagul A Et al.
    Cell metabolism
  27. Decoding the IGF1 signaling gene regulatory network behind alveologenesis from a mouse model of bronchopulmonary dysplasia
    Authors: Feng Gao, Changgong Li, Susan M Smith, Neil Peinado, Golenaz Kohbodi, Evelyn Tran et al.
    eLife
  28. Increased activity of mesenchymal ALK2‐BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues
    Authors: Mohamed Ishan, Guiqian Chen, Chenming Sun, Shi‐You Chen, Yoshihiro Komatsu, Yuji Mishina et al.
    genesis

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