Mouse Kirrel2/NEPH3 Biotinylated Antibody

Catalog #: BAF2930 Datasheet / COA / SDS

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BAF2930

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Mouse Kirrel2/NEPH3 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Kirrel2/NEPH3 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human Kirrel2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kirrel2/NEPH3 isoform 1
Ser19-Asp501
Accession # Q7TSU7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Kirrel2 (Catalog # 2930-K2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel2/NEPH3

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1‑9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589‑598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35‑37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501‑533), while another encodes only portions of Ig domains 1‑4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).

References

Sun C. et al. (2003) Genomics 82:130.
Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
Sellin, L. et al. (2003) FASEB J. 17:115.
SwissProt Accession # Q7TSU7.
Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
Serizawa, S. et al. (2006) Cell 127:1057.
Minaki, Y. et al. (2005) Neurosci. Res. 52:250.

Long Name

Kin of Irregular Chiasm-like 2, Splice Variant A

Entrez Gene IDs

84063 (Human); 243911 (Mouse)

Alternate Names

DKFZp564A1164; Filtrin; FILTRINkin of IRRE-like protein 2; kin of IRRE like 2 (Drosophila); Kin of irregular chiasm-like protein 2; Kirrel2; MGC15718; NEPH3; NEPH3Nephrin-like protein 3; nephrin-like 3; nephrin-like gene 1; NLG1; NLG1kin of irregular chiasm-like 2