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Mouse Klre-1 Antibody

Catalog #: AF7099 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7099
AF7099-SP
Detection of Klre‑1 in Mouse Splenocytes by Flow Cytometry.
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Mouse Klre-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Klre-1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human NKG2D and recombinant mouse NKG2D is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Klre-1
Lys94-Lys226
Accession # NP_705818
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Klre-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of Klre‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Klre-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7099) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Anti-Mouse NK1.1 PE-conjugated antibody. Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klre-1

NKG2I (NKG2 family member I; also Klre-1) is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, NKG2I inhibits NK cell cytotoxic activity. When complexed to KLRI2, NKG2I activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although NKG2I preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse NKG2I is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse NKG2I shares 82% aa identity with rat NKG2I. There does not appear to be a human structural ortholog to mouse NKG2I.

Long Name
Killer Cell Lectin-like Receptor Family E Member 1
Entrez Gene IDs
243655 (Mouse)
Alternate Names
CD94; Klre1; Klre-1; NKG2I

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