Mouse KLRG1 Antibody

Catalog #: MAB6944 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB6944
MAB6944-SP

Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry.

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Mouse KLRG1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse KLRG1 in flow cytometry.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1151A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived mouse KLRG1
Glu57-Tyr188
Accession # O88713

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB6944) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLRG1

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8⁺, CD4⁺, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8⁺ T cells in the elderly and by virus‑specific CD8⁺ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

References

Henson, S.M. and A.N. Akbar (2009) Age 31:285.
Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
Voehringer, D. et al. (2001) Immunogenetics 52:206.
Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
Voehringer, D. et al. (2002) Blood 100:3698.
Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
Thimme, R. et al. (2005) J. Virol. 79:12112.
Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
Ito, M. et al. (2006) J. Exp. Med. 203:289.
Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
Banh, C. et al. (2009) Blood 114:5299.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Alternate Names

2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600