Mouse LAG-3 Antibody

Catalog #: MAB33281 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB33281
MAB33281-SP

Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LAG-3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.

Source

Monoclonal Rat IgG_2B Clone # 631501

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with mouse LAG-3
Ser23-Leu442
Accession # Q61790

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca²⁺Ionomycin then stained with Rat Anti-Mouse LAG-3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113).

Flow Cytometry

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Detection of LAG‑3 in CTLL‑2 mouse cytotoxic T cell line by Flow Cytometry CTLL‑2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG‑3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.

Long Name

Lymphocyte-activation Gene 3

Entrez Gene IDs

3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)

Alternate Names

CD223 antigen; CD223; LAG3; LAG-3; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG-3