Mouse Laminin-1 Antibody

Name: Mouse Laminin-1 Antibody
Brand: R&D Systems
SKU: MAB2549
Rating: 5 (2 reviews)

Catalog #: MAB2549
3 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2549
MAB2549-SP

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Laminin-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Reacts with mouse Laminin-1 A and B chains on the long arm below the cross region.

Source

Monoclonal Rat IgG₁ Clone # AL-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

Immunohistochemistry

8-25 µg/mL

See below

Adhesion Blockade

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Laminin‑1 in Mouse Embryo. Laminin-1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Mouse Laminin-1 Monoclonal Antibody (Catalog # MAB2549) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 637-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival.

Alternate Names

LAMA; LAMA1; laminin subunit alpha 1; Laminin1; Laminin-1; Laminin-111; PTBHS; S-LAM-alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Laminin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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