Mouse Laminin-1 Antibody

Name: Mouse Laminin-1 Antibody
Brand: R&D Systems
SKU: MAB25491
Rating: 5 (2 reviews)

Catalog #: MAB25491 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB25491
MAB25491-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Laminin-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Laminin‑1 in Western blots. This antibody reacts with mouse Laminin-1 beta chain (1).

Source

Monoclonal Rat IgG₁ Clone # AL-3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Laminin‑1 in Mouse Embryo. Laminin-1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Rat Anti-Mouse Laminin-1 Monoclonal Antibody (Catalog # MAB25491) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival (1, 2).

References

Skubitz, A.P. et al. (1988) J Biol Chem. 263:4861
Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.

Alternate Names

LAMA; LAMA1; laminin subunit alpha 1; Laminin1; Laminin-1; Laminin-111; PTBHS; S-LAM-alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Mouse Laminin-1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Mouse Laminin-1 Antibody

By Anonymous on 07/21/2021

Application: IHC Sample Tested: Kidney tissue Species: Mouse

Kidney sections were stained with anti-Laminin antibody (red) to serve as a marker for the basement membrane.

Mouse Laminin-1 Antibody

By Anonymous on 07/02/2021

Application: IHC Sample Tested: Lung tissue Species: Mouse