Mouse Laminin alpha 4 Antibody Summary
Gln826-Ala1816 (predicted)
Accession # P97927
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Laminin alpha 4 in Mouse Stomach. Laminin a4 was detected in perfusion fixed frozen sections of mouse stomach using Rat Anti-Mouse Laminin a4 Monoclonal Antibody (Catalog # MAB3837) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in gastric pits. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 4
Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha, beta, and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin alpha 4, respectively.
Product Datasheets
Citations for Mouse Laminin alpha 4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotype
Authors: V Lam, R Takechi, MJ Hackett, R Francis, M Bynevelt, LM Celliers, M Nesbit, S Mamsa, F Arfuso, S Das, F Koentgen, M Hagan, L Codd, K Richardson, B O'Mara, RK Scharli, L Morandeau, J Gauntlett, C Leatherday, J Boucek, JCL Mamo
PloS Biology, 2021-09-14;19(9):e3001358.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
The lymph node stromal laminin alpha 5 shapes alloimmunity
Authors: L Li, MW Shirkey, T Zhang, Y Xiong, W Piao, V Saxena, C Paluskievi, YS Lee, N Toney, BM Cerel, Q Li, T Simon, KD Smith, KL Hippen, BR Blazar, R Abdi, JS Bromberg
J. Clin. Invest., 2020-05-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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