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Mouse LAMP-1/CD107a Lumenal Domain Antibody

Catalog #: AF4320
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4320
AF4320-SP
Detection of Mouse LAMP‑1/CD107a by Western Blot.
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Product Details
Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LAMP-1/CD107a Lumenal Domain Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human LAMP1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LAMP1/CD107a
Leu25-Asn370
Accession # P11438
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse LAMP-1/CD107a antibody by Western Blot. View Larger

Detection of Mouse LAMP‑1/CD107a by Western Blot. Western blot shows lysates of mouse small intestine tissue and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAMP-1/CD107a at approximately 100-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of LAMP-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of LAMP-1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunocytochemistry LAMP1/CD107a antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

LAMP1/CD107a in RAW 264.7 Mouse Cell Line. LAMP1/CD107a was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane glycoprotein 1; also P2B and CD107a) is a 120 kDa member of the LAMP family of proteins. It is a highly glycosylated type I transmembrane protein whose N-terminus projects either extracellularly or into the lumen of lysosomes. LAMP-1 may protect lysosomal membranes from enzymatic attack or mediate fusion of phagosomes with lysosomes. Mature mouse LAMP1 is 382 amino acids (aa) in length. It contains a 346 aa lumenal domain (aa 25‑370) and a 12 aa cytoplasmic region. Over aa 25‑370, mouse LAMP1 shares 82% and 62% aa identity with rat and human LAMP1, respectively.

Long Name
Lysosome-associated Membrane Glycoprotein 1
Entrez Gene IDs
3916 (Human); 16783 (Mouse)
Alternate Names
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1

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Citations for Mouse LAMP-1/CD107a Lumenal Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Role of liensinine in sensitivity of activated macrophages to ferroptosis and in acute liver injury
    Authors: Jing Li, Qi Huang, Minling Lv, Wenfeng Ma, Jialing Sun, Xin Zhong et al.
    Cell Death Discovery
  2. Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie the Allan-Herndon-Dudley syndrome
    Authors: Salas-Lucia, F;Escamilla, S;Bianco, AC;Dumitrescu, A;Refetoff, S;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  3. Axonal T3 uptake and transport can trigger thyroid hormone signaling in the brain
    Authors: Salas-Lucia F, Fekete C, Sinkó R et al.
    eLife
  4. Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival
    Authors: J Watson, HR Ferguson, RM Brady, J Ferguson, P Fullwood, H Mo, KH Bexley, D Knight, G Howell, JM Schwartz, MP Smith, C Francavill
    Nature Communications, 2022-11-03;13(1):6589.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Ral GTPases are critical regulators of spinal cord myelination and homeostasis
    Authors: J DeGeer, AL Datwyler, C Rickenbach, A Ommer, D Gerber, C Fimiani, J Gerber, JA Pereira, U Suter
    Cell Reports, 2022-09-27;40(13):111413.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  6. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
    Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
    Nature Communications, 2022-03-03;13(1):1134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis
    Authors: Annangi B, Lu Z, Bruniaux J et al.
    Particle and Fibre Toxicology
  8. Microglia prevent beta-amyloid plaque formation in the early stage of an Alzheimer's disease mouse model with suppression of glymphatic clearance
    Authors: W Feng, Y Zhang, Z Wang, H Xu, T Wu, C Marshall, J Gao, M Xiao
    Alzheimers Res Ther, 2020-10-02;12(1):125.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea
    Authors: S Pal-Ghosh, G Tadvalkar, BA Karpinski, MA Stepp
    Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity
    Authors: Olivia Majer, Bo Liu, Lieselotte S. M. Kreuk, Nevan Krogan, Gregory M. Barton
    Nature
  11. Release from UNC93B1 reinforces the compartmentalized activation of select TLRs
    Authors: Olivia Majer, Bo Liu, Brian J. Woo, Lieselotte S. M. Kreuk, Erik Van Dis, Gregory M. Barton
    Nature
  12. Lgl1 controls NG2 endocytic pathway to regulate oligodendrocyte differentiation and asymmetric cell division and gliomagenesis
    Authors: Mathieu Daynac, Malek Chouchane, Hannah Y. Collins, Nicole E. Murphy, Noemi Andor, Jianqin Niu et al.
    Nature Communications
  13. Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7
    Authors: M Koike, M Shibata, T Sunabori, J Yamaguchi, K Sakimura, M Komatsu, K Tanaka, Y Uchiyama
    Am. J. Pathol., 2017-05-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A
    Authors: J Zhang, JL Johnson, J He, G Napolitano, M Ramadass, C Rocca, WB Kiosses, C Bucci, Q Xin, E Gavathioti, AM Cuervo, S Cherqui, SD Catz
    J. Biol. Chem., 2017-05-02;292(25):10328-10346.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles
    Authors: Cyrill Bussy, Erwan Paineau, Julien Cambedouzou, Nathalie Brun, Claudie Mory, Barbara Fayard et al.
    Particle and Fibre Toxicology
  16. Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1.
    Authors: Pal-Ghosh S, Tadvalkar G, Stepp MA.
    Invest. Ophthalmol. Vis. Sci.
  17. Frontotemporal dementia nonsense mutation of progranulin rescued by aminoglycosides
    Authors: Kuang L, Hashimoto K, Huang EJ et al.
    Hum. Mol. Genet.

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