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Mouse Legumain/Asparaginyl Endopeptidase Antibody

Catalog #: AF2058
13 Citations Datasheet / COA / SDS
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AF2058
AF2058-SP
Detection of Mouse Legumain/Asparaginyl Endopeptidase by Western Blot.
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Citations (13)
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Mouse Legumain/Asparaginyl Endopeptidase Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Legumain in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Legumain
Val18-Tyr435
Accession # O89017
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Legumain/Asparaginyl Endopeptidase antibody by Western Blot. View Larger

Detection of Mouse Legumain/Asparaginyl Endopeptidase by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2058) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Legumain/Asparaginyl Endopeptidase at approximately 37 and 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Legumain/Asparaginyl Endopeptidase

Legumain is a lysosomal cysteine protease found in all mouse tissues examined, but was particularly abundant in kidney and placenta (1). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (2). Over-expression of Legumain in tumors is significant for invasion/metastasis (3). Also known as asparaginyl endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of cleavage, which results in the removal of the pro peptides in both C- and N-termini (4). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (5-7).

References
  1. Chen, J.M. et al. (1998) Biochem. J. 335:111.
  2. Shirahama-Noda, K. et al. (2003) J. Biol. Chem. 278:33194.
  3. Liu, C. et al. (2003) Cancer Res. 63: 2957.
  4. Li D.N. et al. (2003) J. Biol. Chem. 278:38980.
  5. Chen, J.M. et al. (2001) Biol. Chem. 382:777.
  6. Schwarz, G. et al. (2002) Biol. Chem. 383:1813.
  7. Sarndeses, C.S. et al. (2003) J. Biol. Chem. 278:13286.
Entrez Gene IDs
5641 (Human); 19141 (Mouse)
Alternate Names
AEP; Asparaginyl Endopeptidase; cysteine protease 1; Legumain; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1EC 3.4.22.34

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Citations for Mouse Legumain/Asparaginyl Endopeptidase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation
    Authors: Cason SE, Mogre SS, Holzbaur ELF, Koslover EF.
    Molecular Biology of the Cell
  2. Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation
    Authors: L Cao, C Xu, P Yi, H Li, Y Lin, G Cai, S Wang, D Ou, M Li, J Chen
    American journal of cancer research, 2021-04-15;11(4):1206-1225.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  3. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI
    Authors: C Chen, D Wang, Y Yu, T Zhao, N Min, Y Wu, L Kang, Y Zhao, L Du, M Zhang, J Gong, Z Zhang, Y Zhang, X Mi, S Yue, X Tan
    Cell Death & Disease, 2021-01-11;12(1):65.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Identification of a new aggressive axis driven by ciliogenesis and absence of VDAC1-deltaC in clear cell Renal Cell Carcinoma patients
    Authors: L Fabbri, M Dufies, S Lacas-Gerv, B Gardie, S Gad-Lapite, J Parola, N Nottet, M Meyenberg, J Contenti, D Borchielli, JM Ferrero, NR Leclercq, D Ambrosetti, B Mograbi, S Richard, J Viotti, E Chamorey, N Sadaghianl, M Rouleau, WJ Craigen, B Mari, S Clavel, G Pagès, J Pouysségur, F Bost, NM Mazure
    Theranostics, 2020-02-03;10(6):2696-2713.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Bilateral Implantation of Shear Stress Modifier in ApoE Knockout Mouse Induces Cognitive Impairment and Tau Abnormalities
    Authors: Shuke Nie, Yang Tan, Zhentao Zhang, Guiqin Chen, Jing Xiong, Dan Hu et al.
    Frontiers in Aging Neuroscience
  6. Involvement of Activation of Asparaginyl Endopeptidase in Tau Hyperphosphorylation in Repetitive Mild Traumatic Brain Injury
    Authors: Wen Hu, Yunn Chyn Tung, Yanchong Zhang, Fei Liu, Khalid Iqbal
    Journal of Alzheimer's Disease
  7. Protease activity of legumain is inhibited by an increase of cystatin E/M in the DJ-1-knockout mouse spleen, cerebrum and heart
    Authors: T Yamane, M Kozuka, Y Yamamoto, Y Nakano, T Nakagaki, I Ohkubo, H Ariga
    Biochem Biophys Rep, 2017-01-05;9(0):187-192.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. 7,8-Dihydroxyflavone Ameliorates Cognitive Impairment by Inhibiting Expression of Tau Pathology in ApoE-Knockout Mice
    Authors: Yang Tan
    Front Aging Neurosci, 2016-11-29;8(0):287.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. The protease cathepsin L regulates Th17 cell differentiation
    Authors: Lifei Hou, Jessica Cooley, Richard Swanson, Poh Chee Ong, Robert N. Pike, Matthew Bogyo et al.
    Journal of Autoimmunity
  10. Loss of Prkar1a leads to Bcl-2 family protein induction and cachexia in mice
    Authors: L Gangoda, M Doerflinger, R Srivastava, N Narayan, L E Edgington, J Orian et al.
    Cell Death & Differentiation
  11. Functional Imaging of Legumain in Cancer Using a New Quenched Activity-Based Probe
    Authors: Laura E. Edgington, Martijn Verdoes, Alberto Ortega, Nimali P. Withana, Jiyoun Lee, Salahuddin Syed et al.
    Journal of the American Chemical Society
  12. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
    Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
    FASEB J., 2010-05-21;24(10):3744-55.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  13. Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
    Authors: Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD
    Atherosclerosis, 2008-02-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P

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