Mouse Lgr5/GPR49 PE-conjugated Antibody

Catalog #: FAB8240P
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8240P-100UG

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry.

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All Lgr5/GPR49 Antibodies

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Citations (3)

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Mouse Lgr5/GPR49 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Lgr5/GPR49 in ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of Lgr5 (Ref. 1).

Source

Monoclonal Rat IgG_2B Clone # 803420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Lgr5/GPR49
Lys375-Pro547
Accession # Q9Z1P4

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Lgr5/GPR49 PE-conjugated Monoclonal Antibody (Catalog # FAB8240P). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5⁺ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).

References

Peng et al. (2013) Cell Rep. 3(6):1885.
de Lau et al. (2014) Genes Dev. 28(4):305.
Barker et al. (2013) Development 140(12):2484.
Clevers (2013) Nature 495(7439):53.
Wu et al. (2014) Nat Commun 5:3149.
Jang et al. (2013) PLoS One 8(12):e82390495.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Alternate Names

FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38

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Citations for Mouse Lgr5/GPR49 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence
Authors: HY Chiang, HH Lu, JN Sudhakar, YW Chen, NS Shih, YT Weng, JW Shui
Nature Communications, 2022-02-15;13(1):874.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/&beta-Catenin Signaling
Authors: Y Li, M Chen, J Hu, R Sheng, Q Lin, X He, M Guo
Cell Stem Cell, 2020-10-13;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation
Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity
Authors: B Kumar, AK Adebayo, M Prasad, ML Capitano, R Wang, P Bhat-Naksh, M Anjanappa, E Simpson, D Chen, Y Liu, JM Schilder, AB Colter, C Maguire, CJ Temm, G Sandusky, EH Doud, AB Wijeratne, AL Mosley, HE Broxmeyer, H Nakshatri
Science Advances, 2022-01-12;8(2):eabh3375.