Mouse Limitin/IFN-zeta Biotinylated Antibody

Catalog #: BAF597 Datasheet / COA / SDS

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BAF597

Detection of Limitin/IFN‑ zeta in Mouse Thymus.

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Mouse Limitin/IFN-zeta Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects Limitin/IFN‑ zeta in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) IFN-gamma and rmIFN-alpha / beta R1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-expressed human LAT
aa 30-113/His
Accession # Q9R1T0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Limitin/IFN‑ zeta (Catalog # 1535-LM)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse thymus

Mouse Limitin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Limitin/IFN-zeta Antibody (Catalog # MAB5971)

Standard: Recombinant Mouse Limitin/IFN-zeta Protein (Catalog # 1535-LM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Limitin/IFN‑ zeta in Mouse Thymus. Limitin/IFN‑ zeta was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse Limitin/IFN‑ zeta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF597) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and B cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Limitin/IFN-zeta

Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.

Long Name

Interferon zeta

Entrez Gene IDs

319146 (Mouse)

Alternate Names

6030405N23Rik; BGIF; Ifnz; IFN-z; IFNzeta; interferon zeta; Limitin; Lmtn