Mouse LRRC32/GARP APC-conjugated Antibody

Name: Mouse LRRC32/GARP APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB62291A

Catalog #: FAB62291A Datasheet / COA / SDS

Discontinued Product

FAB62291A has been discontinued.

View all LRRC32/GARP products.

Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry.

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All LRRC32/GARP Antibodies

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Mouse LRRC32/GARP APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) LRRC32 is observed and no cross-reactivity with rhLRRC3, rhLRRC4, or rhNGL-3/LRRC4B is observed.

Source

Monoclonal Rat IgG₁ Clone # 725226

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP
Ile18-Asn628
Accession # NP_001106850

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stimulated with anti-CD3/anti-CD28, Recombinant Mouse IL-2 (Catalog # 402-ML) and TGF-beta for 3 days to induce regulatory T cell differentiation. Cells were stained with Rat Anti-Mouse CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rat Anti-Mouse LRRC32/GARP APC-conjugated Monoclonal Antibody (Catalog # FAB62291A) or (B) Rat IgG₁Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LRRC32/GARP

Leucine-rich Repeat Protein 32 (LRRC32), also known as GARP (Glycoprotein A Repetitions Predominant), is an 77-80 kDa type I transmembrane glycoprotein. Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, followed by a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain. Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is expressed on hepatic stellate cells and on adult platelets. Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg). LRRC32 expression promotes the acquisition of a Treg phenotype that is characterized by reduced cellular proliferation and cytokine secretion, plus the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and, in association with alpha V beta 8 Integrin, mediates the activation and release of TGF-beta from the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

Long Name

Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant

Entrez Gene IDs

2615 (Human); 434215 (Mouse)

Alternate Names

D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32