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Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

Catalog #: BAM1037
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BAM1037
Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.
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Citations (1)
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Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
Source
Monoclonal Rat IgG2B Clone # RB6-8C5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
Nitta, H. et al. (1997) Cell Vision 4:73.
 
Immunoprecipitation
Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Ly-6G/Ly-6C (Gr-1) antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

Ly-6G/Ly-6C (Gr-1) in Mouse Spleen. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly-6G/Ly-6C (Gr-1)

Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References
  1. Spangrude, G.J. et al. (1988) Science 241:58
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399
  3. Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
  4. Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139
Long Name
A Myeloid Differentiation Antigen
Entrez Gene IDs
546644 (Mouse)
Alternate Names
Gr1; Gr-1; Ly-6C; Ly6G; Ly-6G; ly-6G.1; Ly-6G/Ly-6C (Gr-1); lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

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Citation for Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Oral chymase inhibitor SUN13834 ameliorates skin inflammation as well as pruritus in mouse model for atopic dermatitis.
    Authors: Terakawa M, Fujieda Y, Tomimori Y, Muto T, Tanaka T, Maruoka H, Nagahira K, Ogata A, Nakatsuka T, Fukuda Y
    Eur. J. Pharmacol., 2008-10-29;601(1):186-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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