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Mouse MARCO Antibody

Catalog #: AF2956
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2956
AF2956-SP
Detection of Mouse MARCO by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MARCO Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MARCO in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse MARCO antibody by Western Blot. View Larger

Detection of Mouse MARCO by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2956) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein that belongs to the class A scavenger receptor family (1). Mouse MARCO consists of a 48 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 449 aa extracellular domain (ECD) that includes a stalk region, a collagen-like region, and one SRCR domain (2). Within the ECD, mouse MARCO shares 69% and 86% aa sequence identity with human and rat MARCO, respectively. It shares 18%‑28% aa sequence identity with other mouse class A scavenger receptors CL-P1, SCARA3, SCARA5, and SR-A1/MSR. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (2, 3). Its expression is induced on Kupffer cells and alveolar macrophages by microbial infection, chemical irritants, and Th1 polarizing factors (3‑5). MARCO binds LPS, lipoteichoic acid, and other determinants on Gram positive and Gram negative bacteria (2, 6‑8). It also binds modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO2 (2, 9‑11). MARCO is required for the organization of the splenic marginal zone and the interaction of local macrophages and B cells (12, 13). The SRCR domain mediates binding of MARCO to its various ligands (3, 12), while the collagen-like region mediates assembly into a disulfide-linked trimeric molecule (2, 7). MARCO ligation induces, but is not required for the production of IL-12, NO, or TNF-alpha by macrophages (5, 6, 9). MARCO knockout mice show a reduced clearance of bacterial infections, reduced mast cell mediated silicosis, increased pulmonary inflammation, and increased sensitivity to ozone induced lung damage (4, 9, 14‑16).

References
  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Elomaa, O. et al. (1995) Cell 80:603.
  3. Van der Laan, L.J.W. et al. (1999) J. Immunol. 162:939.
  4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
  5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032. 
  6. Mukhopadhyay, S. et al. (2006) Eur. J. Immunol. 36:940. 
  7. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. 
  8. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767. 
  9. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
  10. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  11. Hamilton, Jr. R.F. et al. (2006) J. Biol. Chem. 281:34218.
  12. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.
  13. Chen, Y. et al. (2005) J. Immunol. 175:8173.
  14. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
  15. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
  16. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
Long Name
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs
8685 (Human); 17167 (Mouse)
Alternate Names
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2

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Citations for Mouse MARCO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Comparative Transcriptomic Analysis Identifies a Range of Immunologically Related Functional Elaborations of Lymph Node Associated Lymphatic and Blood Endothelial Cells
    Authors: Stella J. Berendam, Alexander F. Koeppel, Nicole R. Godfrey, Sherin J. Rouhani, Amber N. Woods, Anthony B. Rodriguez et al.
    Frontiers in Immunology
  2. Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria
    Authors: Z Li, Y Jiao, EK Fan, MJ Scott, Y Li, S Li, TR Billiar, MA Wilson, X Shi, J Fan
    J. Immunol., 2017-09-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Role of lysosomes in silica-induced inflammasome activation and inflammation in absence of MARCO.
    Authors: Biswas R, Hamilton R, Holian A
    J Immunol Res, 2014-06-26;2014(0):304180.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Role of Macrophage Receptor with Collagenous Structure in Innate Immune Tolerance
    Authors: Jian Jing, Ivana V. Yang, Lucy Hui, Jay A. Patel, Christopher M. Evans, Rytis Prikeris et al.
    The Journal of Immunology
  5. Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma.
    Authors: Yu M, Eckart MR, Morgan AA, Mukai K, Butte AJ, Tsai M, Galli SJ
    J. Clin. Invest., 2011-08-01;121(8):3133-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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