Mouse Mast Cell Protease-1/Mcpt1 Antibody

Catalog # Availability Size / Price Qty
MAB5146
MAB5146-SP
Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot.
2 Images
Product Details
Citations (2)
FAQs
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Mouse Mast Cell Protease-1/Mcpt1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mast Cell Protease‑1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCP-6, -7, or ‑11 is observed.
Source
Monoclonal Rat IgG2B Clone # 285008
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
Ile21-Lys246
Accession # P11034
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Mast Cell Protease-1/Mcpt1 antibody by Western Blot. View Larger

Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Mast Cell Protease-1/Mcpt1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-1/Mcpt1

Mast cell protease-1 (MCPT-1), also known as beta -chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMCPT-1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha -Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

References
  1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
  2. Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
  3. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
  4. Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
  5. Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
  6. Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.
Entrez Gene IDs
17224 (Mouse); 29265 (Rat)
Alternate Names
AV080368; Mast Cell Protease1; Mast Cell Protease-1; Mcp1; Mcp-1; MMPC-1

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Citations for Mouse Mast Cell Protease-1/Mcpt1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells
    Authors: Joris van der Veeken, Clarissa Campbell, Yuri Pritykin, Michail Schizas, Jacob Verter, Wei Hu et al.
    Immunity
  2. PTH Promotes Allograft Integration in a Calvarial Bone Defect
    Authors: Dmitriy Sheyn, Doron Cohn Cohn Yakubovich, Ilan Kallai, Susan Su, Xiaoyu Da, Gadi Pelled et al.
    Molecular Pharmaceutics

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