Mouse Mast Cell Protease-1/Mcpt1 Antibody Summary
Ile21-Lys246
Accession # P11034
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Mast Cell Protease‑1/Mcpt1 by Simple WesternTM. Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mast Cell Protease-1/Mcpt1
Mast cell protease-1 (MCPT-1), also known as beta -chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMCPT-1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha -Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.
- Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
- Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
- Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
- Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
- Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
- Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.
Product Datasheets
Citations for Mouse Mast Cell Protease-1/Mcpt1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells
Authors: Joris van der Veeken, Clarissa Campbell, Yuri Pritykin, Michail Schizas, Jacob Verter, Wei Hu et al.
Immunity
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PTH Promotes Allograft Integration in a Calvarial Bone Defect
Authors: Dmitriy Sheyn, Doron Cohn Cohn Yakubovich, Ilan Kallai, Susan Su, Xiaoyu Da, Gadi Pelled et al.
Molecular Pharmaceutics
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