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Mouse Mast Cell Protease-11/Prss34 Antibody

Catalog #: MAB2857 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2857
MAB2857-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mast Cell Protease-11/Prss34 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.
Source
Monoclonal Rat IgG1 Clone # 451421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # Q80UR4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-11/Prss34

Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The amino acid sequence of mouse MCP-11 shares 84% and 60% identity with that of rat and dog, respectively. The human gene corresponding to Prss34 contains a mutation that leads to a premature translation termination codon, thus encoding a protein that is unlikely to be enzymatically active.

References
  1. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
Entrez Gene IDs
328780 (Mouse)
Alternate Names
EOS; Mast Cell Protease11; Mast Cell Protease-11; MCP-11; MCPT11; Prss34

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