Mouse Mast Cell Protease-6/Mcpt6 Antibody

Catalog #: MAB4288 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4288
MAB4288-SP

All Mast Cell Protease-6/Mcpt6 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mast Cell Protease-6/Mcpt6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 286828

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
Ile32-Ser276
Accession # P21845

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (1 µg/mL, Catalog # 3736-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (10 µM, Catalog # ES002). The Neutralization Dose (ND₅₀) is typically 3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast Cell Protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

References

Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535.
Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.

Entrez Gene IDs

7177 (Human); 17229 (Mouse); 29268 (Rat)

Alternate Names

Mast Cell Protease6; Mast Cell Protease-6; Mcpt6

Product Datasheets

Product Datasheet

COA

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