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Mouse Matrilin-3 Antibody

Catalog #: AF3357
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3357
AF3357-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Matrilin-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Matrilin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Matrilin-3 is observed and 5% cross-reactivity with recombinant mouse (rm) Matrilin-2 and rmMatrilin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Matrilin-3
Ala35-Arg481
Accession # AAH71224
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Matrilin‑3 (Catalog # 3357-MN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Matrilin-3

Matrilin-3 is a 50‑60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix (1‑3). The mouse Matrilin-3 cDNA encodes a 481 amino acid (aa) precursor with a 27 aa signal sequence, an N-terminal VWA domain, four tandem EGF-like repeats, and a C-terminal coiled-coil domain (4). The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Mouse Matrilin-3 shares 82% aa sequence identity with human Matrilin-3. Within the first VWA domain, mouse Matrilin-3 shares approximately 51% aa sequence identity with mouse Matrilin-1, -2, and -4. The coiled-coil domain of Matrilin-3 mediates disulfide-linked homo-oligomerization, with tetramer formation being the most dominant (5‑7). It can also assemble into hetero-oligomers with Matrilin-1 (5‑7). Matrilin-3 is more plentiful than Matrilin-1 in the proliferative zone of the growth plate, whereas the reverse is true in the maturation zone (5). Matrilin-3 interacts directly with Collagen IX and COMP (8, 9). In the absence of Collagen IX, the expression of Matrilin-3 is unchanged, although it is retained inside chondrocytes and is not incorporated into the matrix (9). Intracellular retention of Matrilin-3 also occurs with particular point mutations in the VWA domain that results in multiple epiphyseal dysplasia (11‑13). In contrast, a point mutation in the first EGF-like repeat which has been linked to hand osteoarthritis does not prevent Matrilin-3 secretion (13). Matrilin-3 knockout mice do not display any obvious abnormalities, suggesting that other molecules may compensate for the lack of Matrilin-3 (10).

References
  1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
  2. Deak, F. et al. (1999) Matrix Biol. 18:55.
  3. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369.
  4. Wagener, R. et al. (1997) FEBS Lett. 413:129.
  5. Zhang, Y. and Q. Chen (2000) J. Biol. Chem. 275:32628.
  6. Klatt, A.R. et al. (2000) J. Biol. Chem. 275:3999.
  7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
  8. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  9. Budde, B. et al. (2005) Mol. Cell. Biol. 25:10465.
  10. Ko, Y. et al. (2004) Mol. Cell. Biol. 24:1691.
  11. Jackson, G.C. et al. (2004) J. Med. Genet. 41:52.
  12. Cotterill, S.L. et al. (2005) Hum. Mutat. 26:557.
  13. Otten, C. et al. (2005) J. Med. Genet. 42:774.
Entrez Gene IDs
4148 (Human); 17182 (Mouse)
Alternate Names
DIPOA; EDM5; HOA; matrilin 3; Matrilin3; Matrilin-3; NDUFC2; OADIP; OS2

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Citations for Mouse Matrilin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
    Authors: KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs
    PLoS Genet., 2019-07-01;15(7):e1008215.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. An inducible cartilage oligomeric matrix protein mouse model recapitulates human pseudoachondroplasia phenotype.
    Authors: Posey KL, Veerisetty AC, Liu P, Wang HR, Poindexter BJ, Bick R, Alcorn JL, Hecht JT
    Am. J. Pathol., 2009-09-17;175(4):1555-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Skeletal abnormalities in mice lacking extracellular matrix proteins, thrombospondin-1, thrombospondin-3, thrombospondin-5, and type IX collagen.
    Authors: Posey KL, Hankenson K, Veerisetty AC, Bornstein P, Lawler J, Hecht JT
    Am. J. Pathol., 2008-05-08;172(6):1664-74.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Mesencephalic astrocyte-derived neurotropic factor is an important factor in chondrocyte ER homeostasis
    Authors: P. A. Bell, E. P. Dennis, C. L. Hartley, R. M. Jackson, A. Porter, R. P. Boot-Handford et al.
    Cell Stress and Chaperones

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