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Mouse MHC class II (I-A/I-E) APC-conjugated Antibody

Catalog #: FAB6118A
2 Citations Datasheet / COA / SDS
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FAB6118A
Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry.
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Citations (2)
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Mouse MHC class II (I-A/I-E) APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MHC class II (I‑A/I‑E) in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # M5/114.15.2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MHC class II (I-A/I-E) antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F) and either (A) Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (Catalog # FAB6118A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MHC class II (I-A/I-E)

The M5/114.15.2 monoclonal antibody recognizes a homologous region of MHC class II associated I-A and I-E antigens (1). It binds a region shared by I-Ab, I-Ad, I-Aq, I-Ed and I-Ek MHC class II antigens, which are expressed on antigen presenting cells (2).

References
  1. Bhattacharya, A. et al. (1981) J. Immunol. 127:2448.
  2. Watts, C. et al. (1997) Ann. Rev. Immunol. 15:821.
Long Name
Major Histocompatibility Complex Class II IA and IE
Entrez Gene IDs
14968 (Mouse)
Alternate Names
E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea; H2-Ea; MHC class II (I-A/I-E); MHC class II IA/IE

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Citations for Mouse MHC class II (I-A/I-E) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation
    Authors: JM Motta, VM Rumjanek, A Mantovani, M Locati
    Biomedicines, 2021-10-04;9(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy� (ADi�), ADi-100
    Authors: DG Alleva, M Rezaee, L Yip, G Ren, J Rosenberg, W Concepcion, A Escher, S Shabahang, AS Thakor
    Biomedicines, 2020-03-04;8(3):.
    Species: Mouse
    Sample Types: Whole Cells

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