Mouse MMP-7 Antibody

Catalog #: MAB7170 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7170
MAB7170-SP

Detection of MMP-7 in bEnd.3 Mouse Cell Line by Flow Cytometry.

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All MMP-7 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MMP-7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MMP-7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-7, recombinant mouse (rm) MMP‑3, or rmMMP-8 is observed.

Source

Monoclonal Rat IgG_2B Clone # 704202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-7
Met1-Leu264
Accession # Q10738

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MMP-7 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

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Detection of MMP-7 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line treated with 25 ng/mL Recombinant Mouse FGF basic (Catalog # 3139-FB) for 24 hours was stained with Rat Anti-Mouse MMP-7 Monoclonal Antibody (Catalog # MAB7170, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-7

MMP-7 (Matrilysin, 28 kDa) is the smallest member of the matrix metalloproteinases (MMP) family. It is expressed by normal and abnormal epithelial cells and is capable of digesting many proteins of the extracellular matrix. MMP-7 is implicated in the activation of Plasminogen, FasL, intestinal alpha-Defensin (important in innate host defense), and MMPs-1, -2, and -9, and in the release of TNF-alpha. Mouse MMP-7 is synthesized with a signal peptide and a propeptide; the preproMMP-7 shows 87% and 70% aa identity with rat and human preproMMP-7, respectively.

Long Name

Matrix Metalloproteinase 7

Entrez Gene IDs

4316 (Human); 17393 (Mouse)

Alternate Names

EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase