Mouse MR1 Antibody

Catalog #: MAB8526 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8526
MAB8526-SP

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Mouse MR1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MR1 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 909417

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MR1
Met1-Arg296
Accession # Q8HWB0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MR1 in Mouse Intestine. MR1 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse MR1 Monoclonal Antibody (Catalog # MAB8526) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to villi epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MR1

The Major Histocompatibility Complex (MHC) class I-related gene, MR1, considered a non-classical MHC class IA gene which is encoded outside the MHC region. The MR1 is responsible for activation of Mucosal-associated Invariant T (MAIT) cells expressing semi-invariant T cell receptors in the presence of bacteria. MR1 is a highly evolutionary conserved protein with regard to alpha 1 and alpha 2 domains corresponding to the peptide-binding domains of classical MHC class I molecules which show about 90% amino acid identity between human and mouse. MR1 protein can associate with beta 2-microglobulin, which indicates that this is a typical class I heterodimer composed of a heavy and a light chain resembling the classical MHC class I molecules.

Long Name

Major Histocompatibility Complex, Class I-Related

Entrez Gene IDs

3140 (Human); 15064 (Mouse)

Alternate Names

Class I histocompatibility antigen-like protein; FLJ31593; H2ls; HLALS; major histocompatibility complex class I-related gene protein; major histocompatibility complex, class I-like sequence; major histocompatibility complex, class I-related; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; MR1