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Mouse MyD88 Antibody

Catalog #: MAB3109
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3109
MAB3109-SP
MyD88 in RAW 264.7 Mouse Cell Line.
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Product Details
Citations (3)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MyD88 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MyD88 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 316902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse MyD88
Met1-Pro296
Accession # Q3U7M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MyD88 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MyD88 in RAW 264.7 Mouse Cell Line. MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse MyD88 Monoclonal Antibody (Catalog # MAB3109) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin-1 receptor (TIR) domain and is classified as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.

Long Name
Myeloid Differentiation Primary Response Gene 88
Entrez Gene IDs
4615 (Human); 17874 (Mouse)
Alternate Names
MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88

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Citations for Mouse MyD88 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Inhibition of MyD88 Signaling Skews Microglia/Macrophage Polarization and Attenuates Neuronal Apoptosis in the Hippocampus After Status Epilepticus in Mice
    Authors: Liu JT, Wu SX, Zhang H et al.
    Neurotherapeutics
  2. HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells.
    Authors: Jiang G, Sun D, Yang H, Lu Q, Kaplan H, Shao H
    J Leukoc Biol, 2013-12-27;95(4):599-607.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells.
    Authors: Mancuso G, Gambuzza M, Midiri A, Biondo C, Papasergi S, Akira S, Teti G, Beninati C
    Nat. Immunol., 2009-06-01;10(6):587-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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