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Mouse Nanog Antibody

Catalog #: AF2729
35 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2729
AF2729-SP
Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry.
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Citations (35)
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Mouse Nanog Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Nanog
Trp154-Leu262
Accession # Q80Z64
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Yang, W. et al. (2014) Nat. Comm. 5:3818
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Nanog antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Immunocytochemistry Nanog antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Nanog by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Nanog by Immunocytochemistry/Immunofluorescence HP1 beta is highly expressed and diffuse in nuclei of pluripotent cells. a Confocal images of MEFs (top), R1 ESCs (middle) and Rr5 iPSCs (bottom) immunostained for Nanog (green, middle), HP1 beta (red, right) and counterstained with DAPI (blue, left). Asterisks indicate examples of MEFs used as a feeder layer in the culture of the pluripotent cells. b Quantification of the fluorescence intensities of Nanog (green bars) and HP1 beta (red bars) for the three cell types (n ≥ 26). Nanog is used as a marker for pluripotent cells; the fluorescence intensity of the background intensity was subtracted. c Number of HP1 beta foci in the different cell types. Error bars in (b) and (c) represent standard error of the mean. d Confocal images of R1 ESCs immunostained for Nanog (green, middle), HP1 gamma (red, right) and counterstained with DAPI (blue, left). e Confocal images of Rr5 iPSCs immunostained for HP1 gamma (red) and Nanog (inset, green). Asterisks indicate feeder layer MEF cells in (d) and (e). Scale bars for (a–e) = 15 μm. f Time lapse spinning disk confocal images of ESCs expressing the endogenous HP1 beta fused to mCherry induced to differentiate with 1 μM of retinoic acid (RA) for 40 hours (see also Additional file 7 for a video) Image collected and cropped by CiteAb from the following publication (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0760-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Nanog by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Nanog by Immunocytochemistry/Immunofluorescence HP1 beta is highly expressed and diffuse in nuclei of pluripotent cells. a Confocal images of MEFs (top), R1 ESCs (middle) and Rr5 iPSCs (bottom) immunostained for Nanog (green, middle), HP1 beta (red, right) and counterstained with DAPI (blue, left). Asterisks indicate examples of MEFs used as a feeder layer in the culture of the pluripotent cells. b Quantification of the fluorescence intensities of Nanog (green bars) and HP1 beta (red bars) for the three cell types (n ≥ 26). Nanog is used as a marker for pluripotent cells; the fluorescence intensity of the background intensity was subtracted. c Number of HP1 beta foci in the different cell types. Error bars in (b) and (c) represent standard error of the mean. d Confocal images of R1 ESCs immunostained for Nanog (green, middle), HP1 gamma (red, right) and counterstained with DAPI (blue, left). e Confocal images of Rr5 iPSCs immunostained for HP1 gamma (red) and Nanog (inset, green). Asterisks indicate feeder layer MEF cells in (d) and (e). Scale bars for (a–e) = 15 μm. f Time lapse spinning disk confocal images of ESCs expressing the endogenous HP1 beta fused to mCherry induced to differentiate with 1 μM of retinoic acid (RA) for 40 hours (see also Additional file 7 for a video) Image collected and cropped by CiteAb from the following publication (https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0760-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency
(1-3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

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Citations for Mouse Nanog Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  2. Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation
    Authors: LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou
    Cell Stem Cell, 2020-06-30;0(0):.
  3. Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation
    Authors: J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas
    Cell Reports, 2023-01-05;42(1):111942.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  5. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels
    Authors: A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A
    Cell Death & Disease, 2021-06-21;12(7):637.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  6. Glutamine independence is a selectable feature of pluripotent stem cells
    Authors: Santosha A. Vardhana, Paige K. Arnold, Bess P. Rosen, Yanyang Chen, Bryce W. Carey, Danwei Huangfu et al.
    Nature Metabolism
  7. Zooming in on Cryopreservation of hiPSCs and Neural Derivatives: A Dual-Center Study Using Adherent Vitrification
    Authors: Johanna Kaindl, Ina Meiser, Julia Majer, Annika Sommer, Florian Krach, Alisa Katsen-Globa et al.
    Stem Cells Translational Medicine
  8. Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming
    Authors: S He, F Wang, Y Zhang, J Chen, L Liang, Y Li, M Zhang, X Yang, H Pang, Y Li, X Huang, D Qin, D Pei, H Sun, H Zheng
    Cell Discov, 2019-02-12;5(0):11.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018-04-05;22(4):543-558.e12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
    Authors: YL Miao, A Gambini, Y Zhang, E Padilla-Ba, WN Jefferson, ML Bernhardt, W Huang, L Li, CJ Williams
    Biol. Reprod., 2018-04-01;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  11. A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3 alpha and GSK3 beta in Regulating Embryonic Stem Cell Fate
    Authors: Xi Chen, Ruizhe Wang, Xu Liu, Yongming Wu, Tao Zhou, Yujia Yang et al.
    Developmental Cell
  12. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  13. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
    Stem Cell Res, 2016-08-04;17(2):222-227.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  14. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch
    Sci Rep, 2016-08-02;6(0):30903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  15. Canonical MicroRNA Activity Facilitates but May Be Dispensable for Transcription Factor-Mediated Reprogramming
    Authors: Zhong Liu, Maria Skamagki, Kitai Kim, Rui Zhao
    Stem Cell Reports
  16. Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation.
    Authors: Friend K, Brooks H, Propson N, Thomson J, Kimble J
    PLoS ONE, 2015-09-25;10(9):e0139076.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  17. Derivation of LIF-independent mouse iPS cells with modified Oct4
    Authors: Hiroyuki Hirai, Meri Firpo, Nobuaki Kikyo
    Stem Cell Research
  18. Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
    Authors: Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T
    J Biol Chem, 2015-07-02;290(37):22782-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. FBXL10 protects Polycomb-bound genes from hypermethylation.
    Authors: Boulard M, Edwards J, Bestor T
    Nat Genet, 2015-04-06;47(5):479-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  20. Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
    Authors: Kranc K, Oliveira D, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Braganca J
    Stem Cells, 2015-03-01;33(3):699-712.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  21. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
    Authors: Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
    Stem Cells Dev, 2014-12-10;24(5):597-609.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  22. MicroRNA-mediated regulation of extracellular matrix formation modulates somatic cell reprogramming
    Authors: Zhonghan Li, Jason Dang, Kung-Yen Chang, Tariq M. Rana
    RNA
  23. The histone H2A deubiquitinase Usp16 regulates embryonic stem cell gene expression and lineage commitment.
    Authors: Yang, Wei, Lee, Yun-Hwa, Jones, Amanda E, Woolnough, Jessica, Zhou, Dewang, Dai, Qian, Wu, Qiang, Giles, Keith E, Townes, Tim M, Wang, Hengbin
    Nat Commun, 2014-05-02;5(0):3818.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates mouse ESC fates.
    Authors: Huang G, Yan H, Ye S, Tong C, Ying Q
    Stem Cells, 2014-05-01;32(5):1149-60.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Generation of transgene-free mouse induced pluripotent stem cells using an excisable lentiviral system.
    Authors: Varga E, Nemes C, Davis R, Ujhelly O, Klincumhom N, Polgar Z, Muenthaisong S, Pirity M, Dinnyes A
    Exp Cell Res, 2014-02-18;322(2):335-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-P
  26. Gbx2, a LIF/Stat3 target, promotes reprogramming to and retention of the pluripotent ground state.
    Authors: Tai, Chih-I, Ying, Qi-Long
    J Cell Sci, 2013-01-23;126(0):1093-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. A kinase inhibitor screen identifies small-molecule enhancers of reprogramming and iPS cell generation
    Authors: Zhonghan Li, Tariq M. Rana
    Nature Communications
  28. Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation
    Authors: Shai Melcer, Hadas Hezroni, Eyal Rand, Malka Nissim-Rafinia, Arthur Skoultchi, Colin L. Stewart et al.
    Nature Communications
  29. Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells
    Authors: Jesse L. Cox, Sunil K. Mallanna, Briana D. Ormsbee, Michelle Desler, Matthew S. Wiebe, Angie Rizzino
    Journal of Cell Science
  30. Short-Term Serum-Free Culture Reveals that Inhibition of Gsk3 beta Induces the Tumor-Like Growth of Mouse Embryonic Stem Cells
    Authors: Yanzhen Li, Tamaki Yokohama-Tamaki, Tetsuya S. Tanaka
    PLoS ONE
  31. Small RNA-mediated regulation of iPS cell generation.
    Authors: Li Z, Yang CS, Nakashima K, Rana TM
    EMBO J., 2011-02-01;30(5):823-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  32. LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
    Authors: Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B
    Nat. Cell Biol., 2010-12-12;13(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Loss of Bright/ARID3a Function Promotes Developmental Plasticity
    Authors: Guangyu An, Cathrine A. Miner, Jamee C. Nixon, Paul W. Kincade, James Bryant, Philip W. Tucker et al.
    Stem Cells
  34. Defining the Window of Germline Genesis In Vitro from Murine Embryonic Stem Cells1
    Authors: Julia C. Young, Vinali L. Dias, Kate L. Loveland
    Biology of Reproduction
  35. Klf5 is involved in self-renewal of mouse embryonic stem cells.
    Authors: Parisi S, Passaro F, Aloia L, Manabe I, Nagai R, Pastore L, Russo T
    J. Cell. Sci., 2008-07-24;121(0):2629-34.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ChIP, ICC, IHC

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