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Human/Mouse Neogenin Antibody

Catalog #: AF1079
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1079
AF1079-SP
Detection of Human and Mouse Neogenin by Western Blot.
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Citations (16)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Neogenin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Neogenin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
Ala42-Ile1033 (Asp442-Leu461 del)
Accession # NP_032710
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2.5-10 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Chicken Netrin-1 (Catalog # 128‑N1) to immobilized Recombinant Mouse Neogenin Fc Chimera (Catalog # 1079‑NE) coated at 5 µg/mL (100 µL/well). At 300 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Neogenin antibody by Western Blot. View Larger

Detection of Human and Mouse Neogenin by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, Jurkat human acute T cell leukemia cell line, and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neogenin at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neogenin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Neogenin in Mouse Embryo. Neogenin was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse Neogenin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Neogenin by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neogenin at approximately 207 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Neogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1079) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1,
‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

References
  1. Keeling, S.L. et al. (1997) Oncogene 15:691.
  2. Hong, K. et al. (1999) Cell 97:927.
  3. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
  5. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.
Entrez Gene IDs
4756 (Human); 18007 (Mouse)
Alternate Names
HsT17534; IGDCC2DKFZp547B146; Immunoglobulin superfamily DCC subclass member 2; NEO; NEO1; neogenin (chicken) homolog 1; neogenin 1; neogenin homolog 1; Neogenin; NGN; NGNDKFZp547A066

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

Citations for Human/Mouse Neogenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. RGMB and neogenin control cell differentiation in the developing olfactory epithelium
    Development, 2016-05-01;143(9):1534-46.
  2. Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells
    Authors: G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi
    International Journal of Molecular Sciences, 2022-10-22;23(21):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  3. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury
    Authors: Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou
    Experimental Eye Research
  4. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling
    Authors: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina et al.
    Cell
  5. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
    Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
    Nat Commun, 2020-10-14;11(1):5171.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  6. Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells
    Authors: T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo
    Nat Commun, 2018-11-27;9(1):5022.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Inhibition of neogenin fosters resolution of inflammation and tissue regeneration
    Authors: M Schlegel, A Körner, T Kaussen, U Knausberg, C Gerber, G Hansmann, HS Jónasdótti, M Giera, V Mirakaj
    J. Clin. Invest., 2018-09-17;0(0):.
    Species: Human, Mouse, Transgenic Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Apoptosis, Efferocytosis Assay, Neutralization, Stimulation, Transcriptional Analysis
  8. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
    Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
    Nat. Neurosci., 2018-04-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats.
    Authors: Frydlova J, Prikryl P, Truksa J, Falke L, Du X, Gurieva I, Vokurka M, Krijt J
    PLoS ONE, 2016-02-04;11(2):e0148540.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Authors: Dianne M. A. van den Heuvel, Anita J. C. G. M. Hellemons, R. Jeroen Pasterkamp
    PLoS ONE
  11. The axonal guidance receptor neogenin promotes acute inflammation.
    Authors: Konig K, Gatidou D, Granja T, Meier J, Rosenberger P, Mirakaj V
    PLoS ONE, 2012-03-06;7(3):e32145.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
    Authors: Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A
    J. Neurosci., 2012-01-11;32(2):411-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis.
    Authors: Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G
    Am. J. Pathol., 2009-08-21;175(3):1010-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
    Authors: Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J
    Proc. Natl. Acad. Sci. U.S.A., 2008-08-21;105(34):12491-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Selective binding of RGMc/hemojuvelin, a key protein in systemic iron metabolism, to BMP-2 and neogenin.
    Authors: Kuns-Hashimoto R, Kuninger D, Nili M, Rotwein P
    Am. J. Physiol., Cell Physiol., 2008-02-20;294(4):C994-C1003.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Interaction of amyloid precursor protein with contactins and NgCAM in the retinotectal system.
    Authors: Osterfield M, Egelund R, Young LM, Flanagan JG
    Development, 2008-02-13;135(6):1189-99.
    Species: Chicken
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Human/Mouse Neogenin Antibody
By Anonymous on 01/25/2019
Application: WB Sample Tested: Brain tissue Species: Mouse

Dilution used for the western is 1:250

Western Blot Human/Mouse Neogenin Antibody AF1079