Mouse Nephrin Biotinylated Antibody

Catalog #: BAF3159
3 Citations Datasheet / COA / SDS

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BAF3159

All Nephrin Antibodies

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Citations (3)

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Mouse Nephrin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Nephrin in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Nephrin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Nephrin
Gln37-Thr1049
Accession # Q9QZS7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Nephrin (Catalog # 3159-NN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephrin

Nephrin is a 185 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse Nephrin consists of a 1042 amino acid (aa) extracellular domain (ECD) with eight Ig-like C2-set domains and one fibronectin type III domain, a 22 aa transmembrane segment, and a 156 aa cytoplasmic tail (2, 3). Within the ECD, mouse Nephrin shares 84% and 95% aa sequence identity with human and rat Nephrin, respectively. Usage of the alternate exon 1B results in a distinct N-terminal sequence that lacks a clearly defined signal peptide cleavage site (4). Nephrin is expressed primarily on podocytes in the renal glomerulus and to a lesser extent in the brain and pancreas (3, 5). The 1B isoform is not expressed in the kidney (4). Nephrin localizes to intercellular junctions between podocyte foot processes where it functions as a homophilic adhesion molecule (2, 6). Nephrin is required for formation and maintenance of the slit diaphragm between these processes (7). It associates with Neph1, podicin, P-cadherin, and multiple scaffolding proteins which couple it to the actin cytoskeleton (8-12). Nephrin expression is required for the anti-apoptotic effect of VEGF on podocytes as well as for the ability of podocytes to upregulate Glut1 and Glut4 glucose transporters in response to insulin (13, 14). Nephrin downregulation contributes to diabetic nephropathy, and Nephrin mutations underlie the lethal congenital nephritic syndrome NPHS1 (5, 15).

References

Kawachi, H. et al. (2006) Nephrology 11:274.
Holzman, L.B. et al. (1999) Kidney Int. 56:1481.
Putaala, H. et al. (2000) J. Am. Soc. Nephrol. 11:991.
Beltcheva, O. et al. (2003) J. Am. Soc. Nephrol. 14:352.
Putaala, H. et al. (2001) Hum. Mol. Genet. 10:1.
Khoshnoodi, J. et al. (2003) Am. J. Pathol. 163:2337.
Ruotsalainen, V. et al. (2000) Am. J. Pathol. 157:1905.
Barletta, G.M. et al. (2003) J. Biol. Chem. 278:19266.
Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
Lehtonen, S. et al. (2004) Am. J. Pathol. 165:923.
Lehtonen, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:9814.
Verma, R. et al. (2006) J. Clin. Invest. 116:1346.
Foster, R.R. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F48.
Coward, R.J. et al. (2007) Diabetes 56:1127.
Cooper, M.E. et al. (2002) Semin. Nephrol. 22:393.

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Alternate Names

CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor

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Citations for Mouse Nephrin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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