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Mouse Neutrophil Elastase/ELA2 Antibody

Catalog #: AF4517
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4517
AF4517-SP
Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Neutrophil Elastase/ELA2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ELA2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neutrophil Elastase/ELA2
Ser27-Arg260
Accession # Q3UP87
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Neutrophil Elastase/ELA2 antibody by Western Blot. View Larger

Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse Neutrophil Elastase/ELA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing.  When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.

Entrez Gene IDs
1991 (Human); 50701 (Mouse)
Alternate Names
Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1

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Citations for Mouse Neutrophil Elastase/ELA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Sex-dependent role of microglia in disulfide high mobility group box 1 protein-mediated mechanical hypersensitivity
    Authors: Nilesh M. Agalave, Resti Rudjito, Alex Bersellini Farinotti, Payam Emami Khoonsari, Katalin Sandor, Yuki Nomura et al.
    Pain
  2. Cathepsin E in neutrophils contributes to the generation of neuropathic pain in experimental autoimmune encephalomyelitis
    Authors: Harada Y, Zhang J, Imari K et al.
    Pain
  3. A TLR–CXCL1 pathway in DRG neurons induces neutrophil accumulation in the DRG and mechanical allodynia in EAE mice
    Authors: Jing Zhang, Yuka Harada, Yoshinori Hayashi
    Scientific Reports

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