Mouse NKG2A/CD159a APC-conjugated Antibody

Catalog #: FAB6867A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6867A-100UG
FAB6867A-025UG

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry.

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All NKG2A/CD159a Antibodies

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Mouse NKG2A/CD159a APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NKG2A/CD159a in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 705829

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse NKG2A/CD159a
Accession # Q9Z202

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB22252G) and either (A) Rat Anti-Mouse NKG2A/CD159a APC-conjugated Monoclonal Antibody (Catalog # FAB6867A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B