Mouse Nope/IGDCC4 Antibody

Catalog #: MAB1394 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1394
MAB1394-SP

All Nope/IGDCC4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Nope/IGDCC4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Nope/IGDCC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse (rm) DCC, rmNeogenin, and rmPunc is observed.

Source

Monoclonal Rat IgG₁ Clone # 261123

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Nope/IGDCC4
Gly22-His953
Accession # Q9EQS9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Nope/IGDCC4 Fc Chimera (Catalog # 1394-NP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nope/IGDCC4

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions. Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin‑type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mNope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mNope and mouse Punc do not share significant aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares approximately 25 aa similarity with mouse DCC and mouse Neogenin. Mouse and human Nope share 91% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6‑8).

References

Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
Fearon et al. (1990) Science 247:49.
Keino-Masu et al. (1996) Cell 87:175.
Hong, K. et al. (1999) Cell 97:927.
Leonardo, E.D. et al. (1997) Nature 386:833.
Kolodziej et al. (1996) Cell 87:197.
Meyerhardt (1997) Oncogene 14:1129.
Vielmetter et al. (1997) Genomics 41:414.

Long Name

Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4

Entrez Gene IDs

57722 (Human); 56741 (Mouse)

Alternate Names

DDM36; hDDM36; IGDCC4; immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily, DCC subclass, member 4; KIAA1628; likely ortholog of mouse neighbor of Punc E11; LOC57722; Neighbor of punc e11; Nope; NOPEFLJ42051; Protein DDM36