Mouse Notch-2 APC-conjugated Antibody

Catalog #: FAB5196A Datasheet / COA / SDS

Discontinued Product

FAB5196A has been discontinued.
View all Notch-2 products.
Detection of Notch‑2 in C2C12 Mouse Cell Line by Flow Cytometry.
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Mouse Notch-2 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity with rhNotch-1, -3, recombinant mouse (rm) Notch-1, rmNotch-3, or rrDLL1 is observed.
Source
Monoclonal Rat IgG1 Clone # 605724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2
Leu26-Val528 (predicted)
Accession # O35516
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Notch-2 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Notch‑2 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Notch-2 APC-conjugated Monoclonal Antibody (Catalog # FAB5196A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Notch-2

Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins.  It is found on multiple cell types and exhibits multiple functions.  On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation.  Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second of which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor.  There are three potential splice variants.  One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713.  Over aa 26-528, mouse Notch-2 shares 96% and 94% aa sequence identity with rat and human Notch-2, respectively.   

Entrez Gene IDs
4853 (Human); 29492 (Rat)
Alternate Names
AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2

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