Mouse NTB-A/SLAMF6 Antibody

Catalog #: MAB3986 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3986
MAB3986-SP

All NTB-A/SLAMF6 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NTB-A/SLAMF6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NTB‑A/SLAMF6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NTB-A or recombinant mouse CD84 is observed.

Source

Monoclonal Rat IgG_2A Clone # 448318

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NTB‑A/SLAMF6
Glu31-Asn239
Accession # Q9ET39

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse NTB-A/SLAMF6 Fc Chimera (Catalog # 3986-NT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTB-A/SLAMF6

NTB-A, also known as Ly108 and SLAMF6, is a 60 kDa type I transmembrane glycoprotein that belongs to the SLAM subgroup of the CD2 family (1). Mature mouse NTB-A consists of a 209 amino acid (aa) ECD with one Ig-like V-type and one Ig-like C2-type domain, a 23 aa transmembrane segment, and an 89 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2). Within the ECD, mouse NTB-A shares 48% and 70% aa sequence identity with human and rat NTB-A, respectively. The ECD of mouse NTB-A shares 20%‑34% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, and SLAM. An alternatively spliced isoform diverges after the second ITSM (2). NTB-A is expressed on the surface of NK, T, and B lymphocytes as well as eosinophils (3‑5). It interacts homophilically through weak associations between the Ig-V type domains (5‑7). NTB-A functions as an activating coreceptor on NK and T cells (3, 5, 6, 8). Tyrosine phosphorylation in the membrane proximal ITSM enables specific association with EAT-2, an interaction that is required for NTB-A mediated cytotoxicity of NK cells (9). Phosphorylation-dependent NTB-A association with SAP is required for full production of NK cell IFN-gamma (5, 9). This interaction is independent of EAT-2 binding and appears to involve the membrane distal ITSM (5, 9). NTB-A deficient mice show weakened Th2 responses and elevated levels of neutrophil-derived inflammatory mediators (10). On B cells, NTB-A modulates immunoglobulin class switching and the balance between tolerance and autoimmunity (5, 11). The isoform with the divergent C-terminal tail is overexpressed in B cells from lupus-prone mice (11).

References

Veillette, A. (2006) Immunol. Rev. 214:22.
Peck, S.R. and H.E. Ruley (2000) Immunogenetics 52:63.
Bottino, C. et al. (2001) J. Exp. Med. 194:235.
Munitz, A. et al. (2005) J. Immunol. 174:110.
Valdez, P.A. et al. (2004) J. Biol. Chem. 279:18662.
Flaig, R.M. et al. (2004) J. Immunol. 172:6524.
Cao, E. et al. (2006) Immunity 25:559.
Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
Eissmann, P. and C. Watzl (2006) J. Immunol. 177:3170.
Howie, D. et al. (2005) J. Immunol. 174:5931.
Kumar, K.R. et al. (2006) Science 312:1665.

Long Name

NK-T-B Antigen/SLAM Family Member 6

Entrez Gene IDs

114836 (Human)

Alternate Names

Activating NK receptor; CD352 antigen; CD352; KALIFLJ50657; Ly108; NK-T-B-antigen; NTBA receptor; NTBA; NTB-A; NTB-AMGC104953; NTBAT- and B-cell antigen; SF2000; SLAM family member 6; SLAMF6