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Mouse Opticin Antibody

Catalog #: AF3547
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3547
AF3547-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Opticin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Opticin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Opticin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Opticin
Ala20-Thr328 (Tyr168Asp)
Accession # Q920A0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Opticin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Opticin

Opticin, also known as Oculoglycan, is an extracellular matrix protein that belongs to the class III subfamily of the small leucine-rich repeat (SLRP) proteoglycan family. It was initially discovered as a protein associated with vitreous humor collagen fibrils and has since been localized also to numerous ocular tissues and fetal liver. Mouse and human Opticin share 73% amino acid sequence identity.

Entrez Gene IDs
26254 (Human); 269120 (Mouse)
Alternate Names
oculoglycan; Oculogylcan; OPT; OPTC; Opticin

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Citations for Mouse Opticin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression
    Authors: J Pang, L Le, Y Zhou, R Tu, Q Hou, D Tsuchiya, N Thomas, Y Wang, Z Yu, R Alexander, M Thexton, B Lewis, T Corbin, M Durnin, H Li, R Ashery-Pad, D Yan, T Xie
    Cell Reports, 2021-01-12;34(2):108603.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Dominant inhibition of lens placode formation in mice.
    Authors: Zhang Y, Burgess D, Overbeek PA, Govindarajan V
    Dev. Biol., 2008-08-26;323(1):53-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
    Authors: M Farnoodian, CM Sorenson, N Sheibani
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.

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