Mouse Osteoactivin/GPNMB Biotinylated Antibody Summary
Lys23-Asn502
Accession # NP_444340
Applications
Mouse Osteoactivin/GPNMB Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Osteoactivin/GPNMB
Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.
- Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
- Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
- Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Product Datasheets
Citations for Mouse Osteoactivin/GPNMB Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury
Authors: Androvic P, Schifferer M, Perez Anderson K et al.
Nature communications
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Intracerebroventricular Treatment with 2-Hydroxypropyl-&beta-Cyclodextrin Decreased Cerebellar and Hepatic Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) Expression in Niemann-Pick Disease Type C Model Mice
Authors: M Fukaura, Y Ishitsuka, S Shirakawa, N Ushihama, Y Yamada, Y Kondo, T Takeo, N Nakagata, K Motoyama, T Higashi, H Arima, Y Kurauchi, T Seki, H Katsuki, K Higaki, M Matsuo, T Irie
International Journal of Molecular Sciences, 2021-01-05;22(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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