Mouse Osteoprotegerin/TNFRSF11B ELISA Kit - Quantikine
Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of mouse OPG spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=9) | 101 | 85-116 |
EDTA Plasma (n=9) | 97 | 88-107 |
Serum (n=11) | 93 | 83-108 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Osteoprotegerin/TNFRSF11B
Osteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone density. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts, and endothelial cells. OPG functions as a decoy receptor for TRANCE/RANK L and TRAIL. OPG regulates the cell surface expression of TRANCE and its ability to stimulate osteoclastogenesis. Dysregulation of OPG/TRANCE interactions can lead to juvenile Paget’s disease, osteoporosis, bone loss, and vascular calcification.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Bone metastatic mammary tumor cell-derived extracellular vesicles inhibit osteoblast maturation via JNK signaling
Authors: Uehara, N;Shibusawa, N;Mikami, Y;Kyumoto-Nakamura, Y;Sonoda, S;Kato, H;Yamaza, T;Kukita, T;
Archives of biochemistry and biophysics
Species: Mouse
Sample Types: Cell Culture Supernates
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Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration
Authors: E Cho, X Che, MJ Ang, S Cheon, J Lee, KS Kim, CH Lee, SY Lee, HY Yang, C Moon, C Park, JY Choi, TH Lee
Elife, 2023-02-03;12(0):.
Species: Mouse
Sample Types: Serum
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Increased UHMWPE Particle-Induced Osteolysis in Fetuin-A-Deficient Mice
Authors: C Polan, C Brenner, M Herten, G Hilken, F Grabellus, HL Meyer, M Burggraf, M Dudda, W Jahnen-Dec, C Wedemeyer, MD Kauther
Journal of functional biomaterials, 2023-01-04;14(1):.
Species: Mouse
Sample Types: Serum
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Ciliary Neurotrophic Factor (CNTF) Inhibits In Vitro Cementoblast Mineralization and Induces Autophagy, in Part by STAT3/ERK Commitment
Authors: J Yong, S Gröger, J von Bremen, S Ruf
International Journal of Molecular Sciences, 2022-08-18;23(16):.
Species: Mouse
Sample Types: Cell Culture Supernates
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The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
Cell Death & Disease, 2022-06-06;13(6):527.
Species: Human
Sample Types: Cell Culture Supernates
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Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells
Authors: M Smeda, KM Galler, M Woelflick, A Rosendahl, C Moehle, B Lenhardt, W Buchalla, M Widbiller
International Journal of Molecular Sciences, 2022-02-27;23(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Increased bone resorption by long-term cigarette smoke exposure in animal model
Authors: JJM Junqueira, JD Lourenço, KR da Silva, V Jorgetti, RP Vieira, AA de Araujo, K De Angelis, AT Correia, LHV Alves, IFLC Tibério, AP Barbosa, FDTQDS Lopes
Heliyon, 2021-12-10;7(12):e08587.
Species: Mouse
Sample Types: Tissue Homogenates
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Osteoblast-specific inactivation of p53 results in locally increased bone formation
Authors: N Liao, T Koehne, J Tuckermann, I Triviai, M Amling, JP David, T Schinke, J Luther
PLoS ONE, 2021-11-18;16(11):e0249894.
Species: Mouse
Sample Types: Serum
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Decreased Trabecular Bone Mass in Col22a1-Deficient Mice
Authors: W Zhao, P Wiedemann, EM Wölfel, M Neven, S Peters, T Imhof, M Koch, B Busse, M Amling, T Schinke, TA Yorgan
Cells, 2021-11-04;10(11):.
Species: Mouse
Sample Types: Serum
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Hydroxycoumarin Scopoletin Inhibits Bone Loss through Enhancing Induction of Bone Turnover Markers in a Mouse Model of Type 2 Diabetes
Authors: EJ Lee, W Na, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, S Park, K Park, YH Kang
Biomedicines, 2021-06-07;9(6):.
Species: Mouse
Sample Types: Plasma
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Schistosome infection promotes osteoclast-mediated bone loss
Authors: W Li, C Wei, L Xu, B Yu, Y Chen, D Lu, L Zhang, X Song, L Dong, S Zhou, Z Xu, J Zhu, X Chen, C Su
PloS Pathogens, 2021-03-18;17(3):e1009462.
Species: Mouse
Sample Types: Serum
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The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis
Authors: M Timmen, H Hidding, M Götte, TE Khassawna, D Kronenberg, R Stange
Sci Rep, 2020-11-25;10(1):20510.
Species: Mouse
Sample Types: Cell Culture Supernates
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Vascular density with optical coherence tomography angiography and systemic biomarkers in low and high cardiovascular risk patients
Authors: MA Hannappe, L Arnould, A Méloux, B Mouhat, F Bichat, M Zeller, Y Cottin, C Binquet, C Vergely, C Creuzot-Ga, C Guenancia
Sci Rep, 2020-10-07;10(1):16718.
Species: Human
Sample Types: Serum
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Local Production of Osteoprotegerin by Osteoblasts Suppresses Bone Resorption
Authors: KM Cawley, NC Bustamante, AG Guha, RS MacLeod, J Xiong, I Gubrij, Y Liu, R Mulkey, M Palmieri, JD Thostenson, JJ Goellner, CA O'Brien
Cell Rep, 2020-09-08;32(10):108052.
Species: Mouse
Sample Types: Serum
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OPG Production Matters Where It Happened
Authors: M Tsukasaki, T Asano, R Muro, NC Huynh, N Komatsu, K Okamoto, K Nakano, T Okamura, T Nitta, H Takayanagi
Cell Rep, 2020-09-08;32(10):108124.
Species: Mouse
Sample Types: Serum
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Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin
Authors: T Velletri, Y Huang, Y Wang, Q Li, M Hu, N Xie, Q Yang, X Chen, Q Chen, P Shou, Y Gan, E Candi, AP Margherita, M Agostini, H Yang, G Melino, Y Shi, Y Wang
Cell Death Differ., 2020-07-21;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Abaloparatide exhibits greater osteoanabolic response and higher cAMP stimulation and beta-arrestin recruitment than teriparatide
Authors: K Sahbani, CP Cardozo, WA Bauman, HA Tawfeek
Physiol Rep, 2019-10-01;7(19):e14225.
Species: Mouse
Sample Types: Serum
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Deficiency of sphingosine-1-phosphate receptor 3 does not affect the skeletal phenotype of mice lacking sphingosine-1-phosphate lyase
Authors: T Heckt, LJ Brylka, M Neven, M Amling, T Schinke
PLoS ONE, 2019-07-17;14(7):e0219734.
Species: Mouse
Sample Types: Serum
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A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment
Authors: J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park
Sci Rep, 2019-06-19;9(1):8726.
Species: Mouse
Sample Types: Cell Culture Supernates
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Circulating osteogenic proteins are associated with coronary artery calcification and increase after myocardial infarction
Authors: AE Pesaro, M Katz, M Liberman, C Pereira, CLP Mangueira, AEZ de Carvalh, KS Carvalho, CH Nomura, M Franken, CV Serrano
PLoS ONE, 2018-08-23;13(8):e0202738.
Species: Mouse
Sample Types: Plasma
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Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss
Authors: J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien
Nat Commun, 2018-07-25;9(1):2909.
Species: Mouse
Sample Types: Plasma
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Loss of RANKL in osteocytes dramatically increases cancellous bone mass in the osteogenesis imperfecta mouse (oim)
Authors: SM Zimmerman, ME Heard-Lips, M Dimori, JD Thostenson, EM Mannen, CA O'Brien, R Morello
Bone Rep, 2018-07-02;9(0):61-73.
Species: Mouse
Sample Types: Bone Marrow Fluid
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Central RANK signalling in NPY neurons alters bone mass in male mice
Authors: NJ Lee, IM Clarke, RF Enriquez, V Nagy, J Penninger, PA Baldock, H Herzog
Neuropeptides, 2018-02-15;0(0):.
Species: Mouse
Sample Types: Serum
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Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss
Authors: N Yang, B Baban, CM Isales, XM Shi
PLoS ONE, 2017-08-03;12(8):e0181133.
Species: Mouse
Sample Types: Serum
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Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2
Authors: PA Hulley, T Bishop, A Vernet, JE Schneider, JR Edwards, NA Athanasou, HJ Knowles
J. Pathol., 2017-05-29;0(0):.
Species: Mouse
Sample Types: Serum
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Muramyl Dipeptide, a Shared Structural Motif of Peptidoglycans, Is a Novel Inducer of Bone Formation through Induction of Runx2
Authors: OJ Park, J Kim, J Yang, CH Yun, SH Han
J. Bone Miner. Res, 2017-04-27;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Disruption of the Cx43/miR21 pathway leads to osteocyte apoptosis and increased osteoclastogenesis with aging
Authors: HM Davis, R Pacheco-Co, EG Atkinson, LR Brun, AR Gortazar, J Harris, M Hiasa, SA Bolarinwa, T Yoneda, M Ivan, A Bruzzaniti, T Bellido, LI Plotkin
Aging Cell, 2017-03-19;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Deletion of FoxO1, 3 and 4 in Osteoblast Progenitors Attenuates the Loss of Cancellous Bone Mass in a Mouse Model of Type 1 Diabetes
J Bone Miner Res, 2016-09-07;0(0):.
Species: Mouse
Sample Types: Plasma
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Silicon Nitride: A Synthetic Mineral for Vertebrate Biology
Sci Rep, 2016-08-19;6(0):31717.
Species: Human
Sample Types: Cell Culture Supernates
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Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts.
Authors: Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U
J Biol Chem, 2015-06-17;290(33):20147-58.
Species: Mouse
Sample Types: Tissue Homogenates
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5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival.
Authors: Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S
J Biol Chem, 2015-02-17;290(15):9377-86.
Species: Mouse
Sample Types: Serum
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Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss in mice.
Authors: Baek, Jong Min, Kim, Ju-Young, Cheon, Yoon-Hee, Park, Sun-Hyan, Ahn, Sung-Jun, Yoon, Kwon-Ha, Oh, Jaemin, Lee, Myeung S
Molecules, 2014-08-05;19(8):11628-44.
Species: Mouse
Sample Types: Serum
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Inhibition of bone remodeling in young mice by bisphosphonate displaces the plasma cell niche into the spleen.
Authors: Teufel S, Grotsch B, Luther J, Derer A, Schinke T, Amling M, Schett G, Mielenz D, David J
J Immunol, 2014-06-04;193(1):223-33.
Species: Mouse
Sample Types: Serum
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Bisphosphonates inhibit osteosarcoma-mediated osteolysis via attenuation of tumor expression of MCP-1 and RANKL.
Authors: Ohba T, Cole H, Cates J, Slosky D, Haro H, Ando T, Schwartz H, Schoenecker J
J Bone Miner Res, 2014-06-01;29(6):1431-45.
Species: Mouse
Sample Types: Cell Culture Supernates
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Effects of neuropeptides and mechanical loading on bone cell resorption in vitro.
Authors: Yoo Y, Kwag J, Kim K, Kim C
Int J Mol Sci, 2014-04-08;15(4):5874-83.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteocytes produce interferon-beta as a negative regulator of osteoclastogenesis.
Authors: Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T
J Biol Chem, 2014-03-07;289(16):11545-55.
Species: Mouse
Sample Types: Cell Culture Supernates
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Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5).
Authors: Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T
J Bone Miner Res, 2013-10-01;28(10):2070-80.
Species: Mouse
Sample Types: Cell Culture Supernates
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Resolvin E1 and chemokine-like receptor 1 mediate bone preservation.
Authors: Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R
J Immunol, 2012-12-14;190(2):689-94.
Species: Mouse
Sample Types: Cell Culture Supernates
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Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myeloma.
Authors: Buckle, Clive He, De Leenheer, Evy, Lawson, Michelle, Yong, Kwee, Rabin, Neil, Perry, Mark, Vanderkerken, Karen, Croucher, Peter Ia
PLoS ONE, 2012-08-29;7(8):e41127.
Species: Mouse
Sample Types: Serum
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Osteoprotection by semaphorin 3A.
Authors: Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H
Nature, 2012-05-03;485(7396):69-74.
Species: Mouse
Sample Types: Serum
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Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
Authors: Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ
Acta Pharmacol. Sin., 2012-03-19;33(4):479-89.
Species: Rat
Sample Types: Serum
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The RANKL distal control region is required for the increase in RANKL expression, but not the bone loss, associated with hyperparathyroidism or lactation in adult mice.
Authors: Onal M, Galli C, Fu Q, Xiong J, Weinstein RS, Manolagas SC, O'Brien CA
Mol. Endocrinol., 2011-12-29;26(2):341-8.
Species: Mouse
Sample Types: Plasma
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Effects of relaxin and estrogens on bone remodeling markers, receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), in rat adjuvant-induced arthritis.
Authors: Ho TY, Santora K, Chen JC, Frankshun AL, Bagnell CA
Bone, 2011-03-16;48(6):1346-53.
Species: Rat
Sample Types: Plasma
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Systemic effects of ulna loading in male rats during functional adaptation.
Authors: Sample SJ, Collins RJ, Wilson AP, Racette MA, Behan M, Markel MD, Kalscheur VL, Hao Z, Muir P
J. Bone Miner. Res., 2010-09-01;25(9):2016-28.
Species: Rat
Sample Types: Plasma
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Increased trabecular bone formation in mice lacking the growth factor midkine.
Authors: Neunaber C, Catala-Lehnen P, Beil FT
J. Bone Miner. Res., 2010-08-01;25(8):1724-35.
Species: Mouse
Sample Types: Serum
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ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
Authors: Sauer AV, Mrak E, Hernandez RJ, Zacchi E, Cavani F, Casiraghi M, Grunebaum E, Roifman CM, Cervi MC, Ambrosi A, Carlucci F, Roncarolo MG, Villa A, Rubinacci A, Aiuti A
Blood, 2009-07-24;114(15):3216-26.
Species: Mouse
Sample Types: Serum
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Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome.
Authors: O'Connor RD, Zayzafoon M, Farach-Carson MC, Schanen NC
Bone, 2009-05-03;45(2):346-56.
Species: Mouse
Sample Types: Serum
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Beta-Arrestin2 regulates RANKL and ephrins gene expression in response to bone remodeling in mice.
Authors: Pierroz DD, Rufo A, Bianchi EN, Glatt V, Capulli M, Rucci N, Cavat F, Rizzoli R, Teti A, Bouxsein ML, Ferrari SL
J. Bone Miner. Res., 2009-05-01;24(5):775-84.
Species: Mouse
Sample Types: Cell Culture Supernates
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Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma.
Authors: Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD
Blood, 2008-02-27;112(1):196-207.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
Circulation, 2008-01-02;117(3):411-20.
Species: Mouse
Sample Types: Plasma
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Activation of the p53 pathway down-regulates the osteoprotegerin expression and release by vascular endothelial cells.
Authors: Secchiero P, Corallini F, Rimondi E, Chiaruttini C, di Iasio MG, Rustighi A, Del Sal G, Zauli G
Blood, 2007-11-13;111(3):1287-94.
Species: Mouse
Sample Types: Serum
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EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
Authors: Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L
J. Biol. Chem., 2007-07-17;282(37):26656-64.
Species: Mouse
Sample Types: Cell Culture Supernates
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B cells and T cells are critical for the preservation of bone homeostasis and attainment of peak bone mass in vivo.
Authors: Li Y, Toraldo G, Li A, Yang X, Zhang H, Qian WP, Weitzmann MN
Blood, 2007-01-03;109(9):3839-48.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
J. Immunol., 2007-01-01;178(1):192-200.
Species: Mouse
Sample Types: Serum
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JunB as a downstream mediator of PTHrP actions in cementoblasts.
Authors: Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK
J. Bone Miner. Res., 2005-11-07;21(2):246-57.
Species: Mouse
Sample Types: Cell Culture Supernates
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Osteoprotegerin in the inner ear may inhibit bone remodeling in the otic capsule.
Authors: Zehnder AF, Kristiansen AG, Adams JC, Merchant SN, McKenna MJ
Laryngoscope, 2005-01-01;115(1):172-7.
Species: Mouse
Sample Types: Serum
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IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
J. Immunol., 2003-03-01;170(5):2655-62.
Species: Mouse
Sample Types: Synovial Fluid
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