Mouse OX40 Ligand/TNFSF4 Antibody Summary
Gln49-Leu198
Accession # P43488
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑2 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Mouse OX40 Ligand/TNFSF4 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050) and sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # MAB484), Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog # 1236-OX) stimulates IL-2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL-2 Quantikine ELISA Kit (Catalog # M2000). Under these conditions, IL-2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB1236). The ND50 is typically 0.01-0.05 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: OX40 Ligand/TNFSF4
OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and mouse OX40L share 46% sequence identity at the amino acid level (1). OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) which is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is upregulated by CD40 ligation (3). Engagement of the OX40‑OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40‑OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40‑OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).
- Baum, P.R. et al. (1994) EMBO J. 13:3992.
- Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
- Stuber, E. et al. (1995) Immunity 2:507.
- Malstrom, V. et al. (2001) J. Immunol. 166:6972.
- Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
- Weinberg, A.D. (2002) Trends Immunol. 23:102.
Product Datasheets
Citations for Mouse OX40 Ligand/TNFSF4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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TSLP-activated dendritic cells induce T helper type 2 inflammation in Aspergillus fumigatus keratitis
Authors: L Sun, C Dai, J Wu, C Chen, X Wu
Exp. Eye Res., 2018-03-14;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.
Authors: Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M
Mucosal Immunol, 2013-11-13;7(4):786-801.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
T-cell polarization depends on concentration of the danger signal used to activate dendritic cells.
Authors: Peters M, Dudziak K, Stiehm M
Immunol. Cell Biol., 2010-02-02;88(5):537-44.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
OX40 expression in neutrophils promotes hepatic ischemia/reperfusion injury
Authors: Hua Jin, Chunpan Zhang, Chengyang Sun, Xinyan Zhao, Dan Tian, Wen Shi et al.
JCI Insight
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CD4+CD25+ Regulatory T Cells Inhibit Natural Killer Cell Hepatocytotoxicity of Hepatitis B Virus Transgenic Mice via Membrane-Bound TGF-beta and OX40
Authors: Yongyan Chen, Rui Sun, Xunyao Wu, Min Cheng, Haiming Wei, Zhigang Tian
Journal of Innate Immunity
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