Mouse PACAP/ADCYAP1 Antibody Summary
Pro26-Leu175
Accession # O70176
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse PACAP/ADCYAP1 by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF Membrane was probed with 1 µg/mL of Human PACAP/ADCYAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PACAP/ADCYAP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PACAP/ADCYAP1
PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha ‑MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25‑175). It undergoes proteolytic processing to generate two C‑terminal amidated peptides termed PACAP-38 (aa 131‑168) and PACAP-27 (aa 131‑157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25‑175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.
Product Datasheets
Citation for Mouse PACAP/ADCYAP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors
Authors: Yuji Kaneko, Julian P. Tuazon, Xunming Ji, Cesario V. Borlongan
Cellular Physiology and Biochemistry
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