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Mouse PCPE-1 Antibody

Catalog #: AF2239
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2239
AF2239-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PCPE-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PCPE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human PCPE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PCPE-1
Gln25-Ala468 (predicted)
Accession # Q61398
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PCPE‑1
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PCPE-1

PCPE-1 is a secreted 55 kDa enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens. PCPE-1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III.

Long Name
Procollagen C-Proteinase Enhancer-1
Entrez Gene IDs
5118 (Human); 18542 (Mouse)
Alternate Names
PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1Type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; Procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein

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Citation for Mouse PCPE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of PCPE-2 as the endogenous specific inhibitor of human BMP-1/tolloid-like proteinases
    Authors: Vadon-Le Goff, S;Tessier, A;Napoli, M;Dieryckx, C;Bauer, J;Dussoyer, M;Lagoutte, P;Peyronnel, C;Essayan, L;Kleiser, S;Tueni, N;Bettler, E;Mariano, N;Errazuriz-Cerda, E;Fruchart Gaillard, C;Ruggiero, F;Becker-Pauly, C;Allain, JM;Bruckner-Tuderman, L;Nyström, A;Moali, C;
    Nature communications
    Species: Mouse, Human
    Sample Types: Protein
    Applications: Western Blot

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