Mouse Periostin/OSF-2 Isoform 2 Antibody

Name: Mouse Periostin/OSF-2 Isoform 2 Antibody
Brand: R&D Systems
SKU: AF2955
Rating: 4.7 (6 reviews)

Catalog #: AF2955
26 Citations 6 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2955
AF2955-SP

Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody.

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Citations (26)

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Reviews (6)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Periostin/OSF-2 Isoform 2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Periostin/OSF‑2 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2
Asn24-Gln811
Accession # Q62009

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize Periostin/OSF‑2-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Periostin/OSF‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Adhesion Mediated by Periostin/OSF‑2 and Neutral-ization by Mouse Periostin/ OSF‑2 Antibody. Recombinant Mouse Periostin/OSF-2 (2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL.

Immunocytochemistry

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Periostin/OSF‑2 in Rat Mesenchymal Stem Cells. Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References

Clout, N.J. and D. Tisi (2003) Structure 11:197.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
Swiss-Prot Accession # Q62009.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Periostin/OSF-2 Isoform 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Matrix metalloproteinase 14 is required for fibrous tissue expansion
Authors: Susan H Taylor, Ching-Yan Chloé Yeung, Nicholas S Kalson, Yinhui Lu, Paola Zigrino, Tobias Starborg et al.
eLife
Early Cardiac Remodeling Promotes Tumor Growth and Metastasis
Authors: Shimrit Avraham, Soraya Abu-Sharki, Rona Shofti, Tali Haas, Ben Korin, Roy Kalfon et al.
Circulation
Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice
Authors: Jessica Krzistetzko, Cyrill Géraud, Christof Dormann, Anna Riedel, Thomas Leibing
International Journal of Molecular Sciences
Single nuclei transcriptomics reveal the differentiation trajectories of periosteal skeletal/stem progenitor cells in bone regeneration
Authors: Perrin, S;Ethel, M;Bretegnier, V;Goachet, C;Wotawa, CA;Luka, M;Coulpier, F;Masson, C;Ménager, M;Colnot, C;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Phenotypical and biochemical characterization of murine psoriasiform and fibrotic skin disease models in Stabilin-deficient mice
Authors: Krzistetzko, J;Géraud, C;Dormann, C;Riedel, A;Leibing, T;
FEBS open bio
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis
Authors: Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C;
Cancers
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
Lgr5-expressing secretory cells form a Wnt inhibitory niche in cartilage critical for chondrocyte identity
Authors: Ruscitto, A;Chen, P;Tosa, I;Wang, Z;Zhou, G;Safina, I;Wei, R;Morel, MM;Koch, A;Forman, M;Reeve, G;Lecholop, MK;Wilson, M;Bonthius, D;Chen, M;Ono, M;Wang, TC;Yao, H;Embree, MC;
Cell stem cell
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Diabetes Induces Cardiac Fibroblast Activation, Promoting a Matrix-Preserving Nonmyofibroblast Phenotype, Without Stimulating Pericyte to Fibroblast Conversion
Authors: L Alex, I Tuleta, A Hanna, NG Frangogian
Journal of the American Heart Association, 2023-03-09;0(0):e027463.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
Periosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury
Authors: Marchand, T;Akinnola, KE;Takeishi, S;Maryanovich, M;Pinho, S;Saint-Vanne, J;Birbrair, A;Lamy, T;Tarte, K;Frenette, PS;Gritsman, K;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse, Mouse
Sample Types: Whole Tissue
Applications: IHC
Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

FGFR3 in Periosteal Cells Drives Cartilage-to-Bone Transformation in Bone Repair
Authors: A Julien, S Perrin, O Duchamp de, C Carvalho, M Bensidhoum, L Legeai-Mal, C Colnot
Stem Cell Reports, 2020-09-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Hox11 expressing regional skeletal stem cells are progenitors for osteoblasts, chondrocytes and adipocytes throughout life
Authors: KM Pineault, JY Song, KM Kozloff, D Lucas, DM Wellik
Nat Commun, 2019-07-18;10(1):3168.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Short-term RANKL exposure initiates a neoplastic transcriptional program in the basal epithelium of the murine salivary gland
Authors: L Hai, MM Szwarc, DM Lonard, K Rajapakshe, D Perera, C Coarfa, M Ittmann, R Fernandez-, JP Lydon
Cytokine, 2019-06-18;123(0):154745.
Species: Human
Sample Types: Tissue Lysates, Whole Tissue
Applications: IHC, Western Blot

New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to ?-adrenergic stimulation-induced cardiac fibrosis
Authors: S Takahara, SI Inoue, S Miyagawa-T, K Matsuura, Y Nakashima, T Niihori, Y Matsubara, Y Saiki, Y Aoki
EBioMedicine, 2019-03-18;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot

Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
Authors: Y Asada, M Okano, W Ishida, S Iwamoto, K Fukuda, T Hirakata, N Tada, A Fukushima, N Ebihara, A Kudo, A Matsuda
Allergol Int, 2018-11-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
Cancer Res., 2018-05-22;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Deletion of the Ste20-like kinase SLK in skeletal muscle results in a progressive myopathy and muscle weakness
Authors: BR Pryce, KN Al-Zahrani, S Dufresne, N Belkina, C Labr?che, G Patino-Lop, J Frenette, S Shaw, LA Sabourin
Skelet Muscle, 2017-02-02;7(1):3.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
Nat Cell Biol, 2016-04-18;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

A mouse model for dominant collagen VI disorders: heterozygous deletion of Col6a3 Exon 16.
Authors: Pan T, Zhang R, Arita M, Bogdanovich S, Adams S, Gara S, Wagener R, Khurana T, Birk D, Chu M
J Biol Chem, 2014-02-22;289(15):10293-307.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Periostin links mechanical strain to inflammation in abdominal aortic aneurysm.
Authors: Yamashita, Osamu, Yoshimura, Koichi, Nagasawa, Ayako, Ueda, Koshiro, Morikage, Noriyasu, Ikeda, Yasuhiro, Hamano, Kimikazu
PLoS ONE, 2013-11-19;8(11):e79753.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization

Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
Lab Invest, 2012-07-23;92(11):1518-26.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
Authors: Xu BJ, Yan W, Jovanovic B
Proteomics, 2010-07-01;10(13):2458-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells.
Authors: Coutu DL, Wu JH, Monette A, Rivard GE, Blostein MD, Galipeau J
J. Biol. Chem., 2008-04-30;283(26):17991-8001.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Cathepsin A inhibition attenuates myocardial infarction-induced heart failure on the functional and proteomic levels
Authors: Agnese Petrera, Johann Gassenhuber, Sven Ruf, Deepika Gunasekaran, Jennifer Esser, Jasmin Hasmik Shahinian et al.
Journal of Translational Medicine

Periostin gene expression in neu-positive breast cancer cells is regulated by a FGFR signaling cross talk with TGF beta /PI3K/AKT pathways
Authors: Cédrik Labrèche, David P. Cook, John Abou-Hamad, Julia Pascoal, Benjamin R. Pryce, Khalid N. Al-Zahrani et al.
Breast Cancer Research