Mouse Periostin/OSF-2 Isoform 2 Biotinylated Antibody

Catalog #: BAF2955 Datasheet / COA / SDS

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BAF2955

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Mouse Periostin/OSF-2 Isoform 2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Periostin/OSF‑2 Isoform 2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human OSF-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Periostin/OSF‑2 Isoform 2
Asn24-Gln811
Accession # Q62009

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2)

Mouse Periostin/OSF-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Periostin/OSF‑2 Antibody (Catalog # MAB2955)

Standard: Recombinant Mouse Periostin/OSF-2 Protein, CF (Catalog # 2955-F2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Periostin/OSF-2

Mouse Periostin, also known as OSF-2 (osteoblast-specific factor 2) is a 170 kDa, secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1‑4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as a 838 amino acid (aa) precursor that contains a 23 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). Based on human OSF-2, the homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four, 130 aa fasciculin type 1 (or FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel 7-stranded beta -wedge with a multiple alpha -helix fold (1, 8). Multiple alternate splice forms are known to exist C-terminal (aa 672‑812) to the four-fold FAS1 repeats. These mature molecules are 760, 761, 787 and 788 aa in length and show block deletions of 54 aa, 27 aa and/or 28 aa (10). The significance of the alternate splice forms is not clear. They do, however, appear to be temporally regulated (6). OSF-2 is known to bind to alpha _v beta ₃ and alpha _v beta ₅ integrins (3). It is synthesized by smooth muscle cells, fibroblasts and osteoblasts (2, 5, 7). Mature mouse OSF-2 shares 98%, 92% and 91% aa identity with rat, canine and human OSF‑2, respectively.

References

Clout, N.J. and D. Tisi (2003) Structure 11:197.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
Swiss-Prot Accession # Q62009.

Long Name

Osteoblast Specific Factor 2

Entrez Gene IDs

10631 (Human); 50706 (Mouse); 361945 (Rat)

Alternate Names

Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52