Mouse Periostin/OSF-2 Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of mouse Periostin spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=6) | 95 | 81-117 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Periostin/OSF-2
Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation. It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth. It is upregulated in many carcinomas. Periostin binds to multiple Integrins and enhances cell adhesion and cell migration. It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis. It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis. Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis. Periostin plays an important role in heart valve development and tissue healing after myocardial infarction. In asthma, it is upregulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse Periostin/OSF-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Phenotypical and biochemical characterization of murine psoriasiform and fibrotic skin disease models in Stabilin-deficient mice
Authors: Krzistetzko, J;Géraud, C;Dormann, C;Riedel, A;Leibing, T;
FEBS open bio
Species: Mouse, Transgenic Mouse
Sample Types: Plasma
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The secretome atlas of two mouse models of progeria
Authors: Quintana-Torres, D;Valle-Cao, A;Bousquets-Muñoz, P;Freitas-Rodríguez, S;Rodríguez, F;Lucia, A;López-Otín, C;López-Soto, A;Folgueras, AR;
Aging cell
Species: Mouse
Sample Types: Plasma
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MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and Repair
Authors: X Gao, X Sun, H Cheng, JJ Ruzbarsky, M Mullen, M Huard, J Huard
International Journal of Molecular Sciences, 2023-01-25;24(3):.
Species: Mouse
Sample Types: Serum
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MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and Repair
Authors: X Gao, X Sun, H Cheng, JJ Ruzbarsky, M Mullen, M Huard, J Huard
International Journal of Molecular Sciences, 2023;24(3):.
Species: Mouse
Sample Types: Serum
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Cardiac Remodeling in the Absence of Cardiac Contractile Dysfunction Is Sufficient to Promote Cancer Progression
Authors: L Awwad, T Goldenberg, I Langier-Go, A Aronheim
Cells, 2022-03-25;11(7):.
Species: Mouse
Sample Types: Serum
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Periostin loss-of-function protects mice from post-traumatic and age-related osteoarthritis
Authors: M Attur, X Duan, L Cai, T Han, W Zhang, ED Tycksen, J Samuels, RH Brophy, SB Abramson, MF Rai
Arthritis Research & Therapy, 2021-04-08;23(1):104.
Species: Mouse
Sample Types: Serum
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Macrophage-secreted granulin supports pancreatic cancer metastasis by inducing liver fibrosis
Authors: SR Nielsen, V Quaranta, A Linford, P Emeagi, C Rainer, A Santos, L Ireland, T Sakai, K Sakai, YS Kim, D Engle, F Campbell, D Palmer, JH Ko, DA Tuveson, E Hirsch, A Mielgo, MC Schmid
Nat Cell Biol, 2016-04-18;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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