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Mouse PIR-B Antibody

Catalog #: MAB2754
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2754
MAB2754-SP
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PIR-B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.
Source
Monoclonal Rat IgG2A Clone # 326414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse PIR-B
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name
Paired-Ig-like Receptor B
Entrez Gene IDs
18733 (Mouse); 65146 (Rat)
Alternate Names
CD85a; HL9; ILT5; ILT-5; leukocyte immunoglobulin like receptor B3; LILRA6; LILRB3; LIR3; LIR-3; PIRB; PIR-B

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Citations for Mouse PIR-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. LILRB2-containing small extracellular vesicles from glioblastoma promote tumor progression by promoting the formation and expansion of myeloid-derived suppressor cells
    Authors: Peitao Wu, Yuhang Guo, Li Xiao, Jiaqi Yuan, Chao Tang, Jun Dong et al.
    Cancer Immunology, Immunotherapy
  2. HACS1 signaling adaptor protein recognizes a motif in the paired immunoglobulin receptor B cytoplasmic domain
    Authors: Jamie J. Kwan, Sladjana Slavkovic, Michael Piazza, Dingyan Wang, Thorsten Dieckmann, Philip E. Johnson et al.
    Communications Biology
  3. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation.
    Authors: Williams, Jesse W, Tjota, Melissa, Clay, Bryan S, Vander Lugt, Bryan, Bandukwala, Hozefa S, Hrusch, Cara L, Decker, Donna C, Blaine, Kelly M, Fixsen, Bethany, Singh, Harinder, Sciammas, Roger, Sperling, Anne I
    Nat Commun, 2013-01-01;4(0):2990.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. SIRP{alpha}/CD172a Regulates Eosinophil Homeostasis.
    Authors: Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M
    J. Immunol., 2011-07-20;187(5):2268-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Classical major histocompatibility complex class I molecules in motoneurons: new actors at the neuromuscular junction.
    Authors: Thams S, Brodin P, Plantman S, Saxelin R, Karre K, Cullheim S
    J. Neurosci., 2009-10-28;29(43):13503-15.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  6. Soluble LILRA3 promotes neurite outgrowth and synapses formation through high affinity interaction with Nogo 66.
    Authors: An H, Brettle M, Lee T et al.
    J Cell Sci

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