Home / PLET-1 / Mouse PLET-1 Antibody

Mouse PLET-1 Antibody

Catalog #: MAB6917
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6917
MAB6917-SP
Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PLET-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PLET-1 in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 700738
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PLET-1
Ala27-Leu216
Accession # Q8VEN2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PLET-1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunohistochemistry PLET-1 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

PLET‑1 in Mouse Kidney. PLET-1 was detected in immersion fixed frozen sections of adult mouse kidney using Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to medulla collecting ducts. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLET-1

PLET-1 (placenta-expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI-anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET-1, which most likely includes amino acids (aa) 28-216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the C‑terminus. PLET-1 is a marker of early thymic progenitor cells and pancreatic duct epithelium.

Long Name
Placenta-expressed Transcript 1
Entrez Gene IDs
76509 (Mouse)
Alternate Names
AgK114; C11orf34; chromosome 11 open reading frame 34; placenta expressed transcript 1; PLET1; PLET-1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Mouse PLET-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Alveolar macrophage-expressed Plet1 is a driver of lung epithelial repair after viral pneumonia
    Authors: Pervizaj-Oruqaj, L;Selvakumar, B;Ferrero, MR;Heiner, M;Malainou, C;Glaser, RD;Wilhelm, J;Bartkuhn, M;Weiss, A;Alexopoulos, I;Witte, B;Gattenlöhner, S;Vadász, I;Morty, RE;Seeger, W;Schermuly, RT;Vazquez-Armendariz, AI;Herold, S;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse PLET-1 Antibody

There are currently no reviews for this product. Be the first to review Mouse PLET-1 Antibody and earn rewards!

Have you used Mouse PLET-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review