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Mouse PlGF-2 Biotinylated Antibody

Catalog #: BAF465
2 Citations Datasheet / COA / SDS
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BAF465
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PlGF-2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PlGF-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with rmVEGF R1 Fc Chimera, rmVEGF R2 Fc Chimera, and rmVEGF R3 Fc Chimera is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 (R&D Systems, Catalog # 465-PL)
Ala24-Pro158
Accession # Q544A5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PlGF‑2 (Catalog # 465-PL)

Mouse PlGF-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse PlGF‑2 Antibody (Catalog # MAB4651)

Standard: Recombinant Mouse PlGF-2 Protein (Catalog # 465-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF-2

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high-affinity to Flt-1, but not to Flk-1/KDR.

References
  1. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
  2. Cao, Y. et al. (1997) Biochem. Biophys. Res. Commun. 235:493.
  3. Ferrara, N. et al. (1997) Endocrin. Rev. 18:4.
Long Name
Placenta Growth Factor 2
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names
OORS; PlGF2; PlGF-2

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Citations for Mouse PlGF-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy
    Authors: M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura
    PLoS Genet., 2019-12-26;15(12):e1008468.
    Species: Mouse
    Sample Types:
    Applications: ELISA Detection
  2. A critical role of placental growth factor in the induction of inflammation and edema formation.
    Authors: Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M
    Blood, 2002-09-05;101(2):560-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development

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